Data regarding the sensibility to proteolysis of a natural apolipoprotein A-I mutant

Data in Brief
Gisela M GaddiMaría Alejandra Tricerri

Abstract

The article shows dataset of the proteolysis of a natural variant of apolipoprotein A-I (apoA-I) with a substitution of a leucine by and arginine in position 60 (L60R), in comparison with the protein with the native sequence (Wt). This information demonstrates the potential of in vitro partial proteolysis experiments as it may be applicable to different approaches in the biophysical field. We have analyzed by different electrophoresis techniques apoA-I variants, quantified the degree of proteolysis after staining and compared the proteolysis efficiency with the computed cleavage patterns. The data shown here clearly strengthen the usefulness of this approach to test protein flexibility, as it may be attained with enzymes which are not expected to modify in vivo this protein but have a well-known digestion pattern. In addition it is appropriate for evaluating protein catabolism, as it is exemplified here by the evidence with metalloproteinase 12 (MMP-12), which is a physiological protease that may elicit the pro-inflammatory processing of this variant within the lesions. We support the work "Structural analysis of a natural apolipoprotein A-I variant (L60R) associated with amyloidosis" (Gaddi, et al., 2020), gaining insights on ...Continue Reading

References

Jan 3, 2003·Protein Expression and Purification·Robert O RyanMichael N Oda
Jun 26, 2003·Nucleic Acids Research·Elisabeth GasteigerAmos Bairoch
Oct 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason L JohnsonChristopher L Jackson
Aug 4, 2011·PloS One·Nahuel A RamellaM Alejandra Tricerri
Jan 28, 2014·Nature Medicine·Ying HuangStanley L Hazen
Apr 29, 2016·Nature Protocols·Piero GiansantiAlbert J R Heck
Dec 10, 2017·Molecular & Cellular Proteomics : MCP·Xiaojing WangBing Zhang
Mar 21, 2020·Archives of Biochemistry and Biophysics·Gisela M GaddiM Alejandra Tricerri

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

Excel
ExPASy
excelAnalyzed
Image Quant
Image J
Peptide Mass

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