Datura stramonium lectin staining of glial associated extracellular material in insect brains

The Journal of Comparative Neurology
I HähnleinG Bicker

Abstract

To investigate how glial cells structure the neuropile of olfactory pathways in the brains of honeybees and locusts, we used a lectin as a carbohydrate specific molecular label. On frozen sections, Datura stramonium lectin (DSL) stained extracellular material which is mainly associated with glial cells. Preadsorption of the DSL with the carbohydrate N, N'-diacetylchitobiose blocked the staining. The location of glial cells was detected by an antiserum against the glial-specific nuclear repo-protein. Lectin-staining surrounded the neuropile of the antennal lobe, axonal projections of olfactory relay neurons, and the mushroom body neuropile. Within the mushroom body neuropile of the bee, DSL-staining was especially intense at the branching sites of the Kenyon cell axons and in the ventral part of the alpha-lobe. The dissection of the various cellular contributions to the lectin-staining in dissociated cell cultures suggested that certain glial cells, but also neuronal somata of the antennal lobe and Kenyon cells of the mushroom bodies express the label. The expression of lectin-staining matures during the pupal development of the bee, whereas in larval stages of the hemimetabolous locust, the staining pattern appears already comp...Continue Reading

References

Aug 1, 1992·Journal of Neurocytology·S Kreissl, G Bicker
Jan 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S S Spicer, B A Schulte
Apr 1, 1991·Trends in Neurosciences·D Schubert
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·G GrenninglohC S Goodman
Jan 1, 1990·Cold Spring Harbor Symposia on Quantitative Biology·S HockfieldH Fryer
Jul 1, 1971·The Histochemical Journal·D E Ashhurst, N M Costin
Jan 1, 1984·Acta histochemica·G BrücknerD Biesold
Dec 10, 1981·The Journal of Comparative Neurology·M J Weiss
Jan 1, 1994·Brain Research. Brain Research Reviews·M R Celio, I Blümcke
Sep 15, 1993·The Journal of Comparative Neurology·G BickerA Hofbauer
Aug 15, 1993·The Journal of Comparative Neurology·J Rybak, R Menzel
Jan 1, 1993·Annual Review of Neuroscience·D A Steindler
Apr 1, 1996·The Journal of Comparative Neurology·I Hähnlein, G Bicker
Jan 1, 1991·Tissue & Cell·J Gascuel, C Masson

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Citations

Jul 16, 2008·Arthropod Structure & Development·Claudia Groh, Wolfgang Rössler
Nov 17, 2009·Brain Structure & Function·Zoltán Serfozo, Károly Elekes
Jul 19, 2002·The Journal of Comparative Neurology·Nicholas J Strausfeld
Sep 25, 2001·Archives of Insect Biochemistry and Physiology·G Bicker
Sep 22, 2011·The Journal of Comparative Neurology·Michael SternGerd Bicker
Jul 6, 2004·The Journal of Comparative Neurology·Nicholas J GibsonLeslie P Tolbert
Jan 12, 2021·Frontiers in Physiology·Gerd Bicker, Michael Stern
Sep 10, 2002·Developmental Biology·Jennifer BonnerTimothy O'Connor
Jan 30, 2010·Progress in Neurobiology·Tara N Edwards, Ian A Meinertzhagen

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