PMID: 9057105Mar 1, 1997Paper

Daunomycin disrupts nuclear assembly and the coordinate initiation of DNA replication in Xenopus egg extracts

Journal of Cellular Biochemistry
F Leng, Gregory H Leno


We have used Xenopus egg extracts to investigate the effects of the antitumor drug daunomycin on DNA replication in vitro. Xenopus sperm nuclei replicated nearly synchronously in our egg extracts, thereby allowing us to determine the effects of the drug on both replication initiation and elongation. Titration experiments demonstrated that daunomycin effectively inhibited replication in the extract, with 50% inhibition at a total drug concentration of 2.7 microM. However, a high concentration of daunomycin (50 microM) also inhibited nuclear envelope assembly, a prerequisite for the initiation of replication in this system. Therefore, to bypass the effects of daunomycin on nuclear envelope assembly, sperm nuclei were preassembled in extract prior to drug addition. Initiation of replication in preassembled nuclei was also inhibited by daunomycin, with 50% inhibition at a drug concentration of 3.6 microM. At low drug concentrations, where replication did occur, the synchrony of initiations within individual nuclei was lost. This drug-induced disruption of initiation events may provide important clues regarding the mechanism(s) by which these events are coordinated in eukaryotic cells. Daunomycin also inhibited replication elongatio...Continue Reading


Feb 1, 1991·The Journal of Cell Biology·G H Leno, R A Laskey
Mar 1, 1990·The Biochemical Journal·A MontecuccoG Ciarrocchi
Apr 1, 1990·Biophysical Chemistry·J B Chaires
Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J BartkowiakZ Darzynkiewicz
Dec 15, 1987·Biochemistry·J B ChairesM J Waring
May 6, 1988·Biochimica Et Biophysica Acta·M GigliM Manfait
Jul 1, 1965·Journal of Molecular Biology·I B Dawid
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P R LangerD C Ward
Jan 18, 1983·Biochemistry·J B ChairesD M Crothers
Feb 1, 1994·Biochemistry·C J RocheD M Crothers
Mar 29, 1996·The Journal of Biological Chemistry·G H LenoR A Laskey
Sep 1, 1994·Trends in Cell Biology·D Lourim, G Krohne

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