DAX-1 expression is regulated during mammary epithelial cell differentiation

Endocrinology
Luisa A HelgueroLars-Arne Haldosén

Abstract

In recent studies, we have found that DAX-1 (dosage-sensitive sex reversal/adrenal hypoplasia congenita critical region on the X chromosome) is expressed in the mouse mammary epithelial cell line HC11. In this study, we focused on the regulation of DAX-1 expression and subcellular localization throughout mouse mammary epithelial cell differentiation and its hormonal regulation in the mouse mammary gland. Proliferating HC11 cells grown in epidermal growth factor (EGF)-containing medium, expressed very low levels of DAX-1 as detected by Western blotting and quantitative real-time PCR, whereas, upon EGF withdrawal and induction of differentiation, DAX-1 expression increased. Inhibition of MAPK pathway with PD 098059 resulted in increased DAX-1 levels even in the presence of EGF. Using confocal microscopy, we showed that DAX-1 cytoplasmic levels increased as cells differentiated. DAX-1 staining was nuclear in luminal cells of mouse mammary glands from 3-month-old virgin mice. A nucleo-cytoplasmic pattern was observed in pseudopregnant mice and a cytoplasmic pattern was found in mammary glands from 6-d lactating mice. The influence of DAX-1 on transcriptional activity of endogenously expressed estrogen receptors alpha (ERalpha) and ...Continue Reading

References

Apr 1, 1982·Endocrinology·G Shyamala, A Ferenczy
May 5, 1998·Molecular and Cellular Biology·P A CrawfordJ Milbrandt
Oct 29, 2000·The Journal of Biological Chemistry·H ZhangE Treuter
Apr 3, 2001·American Journal of Human Genetics·B K JordanE Vilain
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·Z J WangJ L Jameson
Mar 5, 2002·Molecular Endocrinology·Elin HolterEckardt Treuter
Jan 23, 2003·Journal of Cell Science·Marc Robinson-RechaviVincent Laudet
May 29, 2003·Molecular and Cellular Endocrinology·A AmsterdamR Seger
May 29, 2003·The Journal of Biological Chemistry·Irina U AgoulnikNancy L Weigel
May 31, 2003·Molecular Endocrinology·Enzo Lalli, Paolo Sassone-Corsi
Jun 5, 2003·Molecular and Cellular Endocrinology·Gunnar MellgrenJohan Lund
Aug 12, 2003·Nature Biotechnology·Ross S Chambers, Stephen Albert Johnston
Nov 8, 2003·Molecular Endocrinology·Malin Hedengran FauldsLars-Arne Haldosén
Jun 16, 2005·The Journal of Clinical Endocrinology and Metabolism·Marie-Claude BattistaNicole Gallo-Payet
Jan 5, 2006·Molecular Pharmacology·Patrick AugereauVincent Cavailles

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Citations

May 18, 2007·BMC Genetics·Christina K HastonMelanie Lafleur
Oct 2, 2008·Endocrine Journal·Yasuhiro NakamuraHironobu Sasano
Jan 5, 2014·Cell Death & Disease·E Lalli
Aug 1, 2009·The American Journal of Pathology·Tomofumi OdaEiichi Morii
Apr 3, 2008·Biochemical and Biophysical Research Communications·Gwang Sik KimHueng-Sik Choi
Jan 9, 2010·Expert Opinion on Therapeutic Targets·Enzo Lalli, Javier Alonso

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