Days weaving the lagging strand synthesis of DNA - A personal recollection of the discovery of Okazaki fragments and studies on discontinuous replication mechanism

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
Tsuneko Okazaki

Abstract

At DNA replication forks, the overall growth of the antiparallel two daughter DNA chains appears to occur 5'-to-3' direction in the leading-strand and 3'-to-5' direction in the lagging-strand using enzyme system only able to elongate 5'-to-3' direction, and I describe in this review how we have analyzed and proved the lagging strand multistep synthesis reactions, called Discontinuous Replication Mechanism, which involve short RNA primer synthesis, primer-dependent short DNA chains (Okazaki fragments) synthesis, primer removal from the Okazaki fragments and gap filling between Okazaki fragments by RNase H and DNA polymerase I, and long lagging strand formation by joining between Okazaki fragments with DNA ligase.

References

Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·F TamanoiT Okazaki
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B K TyeB Weiss
Nov 1, 1978·Nucleic Acids Research·K Shinozaki, T Okazaki
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·T OkazakiT Hozumi
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K SugimotoT Okazaki
Aug 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·K SugimotoR Okazaki
Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R SchekmanA Kornberg
Nov 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D BrutlagA Kornberg
Feb 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R OkazakiA Sugino
Dec 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·T Okazaki, R Okazaki
Aug 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·K SugimotoR Okazaki
May 1, 1952·The Journal of General Physiology·A D HERSHEY, M CHASE
Jun 15, 2005·Annual Review of Biochemistry·Aaron Johnson, Mike O'Donnell
Jul 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·M Meselson, F W Stahl

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Citations

Nov 8, 2017·Proceedings of the National Academy of Sciences of the United States of America·Santa S NaoremHuatao Guo
Jan 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Glen E CronanAndrei Kuzminov
Feb 17, 2019·Proceedings of the National Academy of Sciences of the United States of America·Peter M Burgers

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Methods Mentioned

BETA
column chromatography

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