DbcAMP regulates adipogenesis in sheep inguinal preadipocytes

Lipids in Health and Disease
Delin KongJuncai Fu

Abstract

The cyclic adenosine-monophosphate (cAMP) pathway is generally recognized as one of the essential pathways for the adipose conversion of rodent preadipocytes in vitro. However, divergent effects of cAMP on adipocyte differentiation have also been reported. Since there is very little data on non-rodent preadipose cells, the aim of the present work was to analyze the effects of one classic activator (dbcAMP) of the cAMP pathway on the proliferation and differentiation of sheep preadipocytes grown. We retrospectively analyzed the regulation of dbcAMP on the proliferation and differentiation of sheep preadipocytes through observation on cell dynamic morphology, drawing on the growth curve, Oil Red O staining and induction of cell differentiation. 1) During first 5 days of treatment, at lower levels of dbcAMP (1 nmol/L to 1 × 104 nmol/L), sheep cells were not increased, but at higher levels (1 × 105 nmol/l to 1 × 106 nmol/l), they were significantly increased (P < 0.05); 2) dbcAMP had the trendency to promote cell differentition, but it was not significant (P > 0.05); 3) treated for 4 days, dbcAMP at the levels of 1 nmol/L, 1 × 104 nmol/L and 1 × 106 nmol/L increased C20:0 abundance (P < 0.05), but other fatty acids had no significa...Continue Reading

References

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Methods Mentioned

BETA
Assay
PCR
electrophoresis

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