DC electrical field-induced c-fos expression and growth stimulation in multicellular prostate cancer spheroids

British Journal of Cancer
H SauerMaria Wartenberg

Abstract

The effects of electrical direct current (DC) field pulses on c-fos expression, growth kinetics and vitality patterns of multicellular tumour spheroids (MCSs) were studied. Monitoring the membrane potential of MCSs by di-8-ANNEPS staining and confocal microscopy during DC electrical field treatment revealed a hyperpolarization at the anode-facing side and a depolarization at the cathode-facing side. When a single 500 V m(-1) electrical field pulse with a duration of 60 s was applied to MCSs (150-350 microm in diameter) an enhancement of the growth kinetics within a period of 6 days post pulse was observed. Whereas the volume doubling time amounted to 4-5 days in control samples, it was reduced to 1-2 days in electropulsed MCSs. At day 6 post pulse the diameter of the necrotic core was significantly smaller than the control. The critical diameter for the first appearance of central necrosis amounted to 350 +/- 50 microm in the control and 450 +/- 50 microm in the electropulsed MCSs. Coincidentally, the proliferating rim was increased to 107 +/- 11 microm in electropulsed MCSs as compared with 60 +/- 6 microm in the control. The growth stimulation may be mediated by the proto-oncogene c-fos as its expression increased by a factor...Continue Reading

Citations

Nov 26, 1999·Journal of Cellular Biochemistry·H SauerM Wartenberg
Apr 28, 2001·Physics in Medicine and Biology·A Molckovsky, B C Wilson
Sep 14, 2010·In Vitro Cellular & Developmental Biology. Animal·J Antonio Heredia-RojasReyes S Taméz-Guerra
May 4, 2011·International Urology and Nephrology·Xenophon K GiannakopoulosAngelos M Evangelou
Sep 20, 2020·International Journal of Molecular Sciences·Fabrizio FontanaPatrizia Limonta

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