DC-specific ICAM-3-grabbing nonintegrin mediates internalization of HIV-1 into human podocytes.

American Journal of Physiology. Renal Physiology
Joanna MikulakPravin C Singhal

Abstract

Human immunodeficiency virus (HIV)-1 has been demonstrated to contribute to the pathogenesis of HIV-associated nephropathy. In renal biopsy studies, podocytes have been reported to be infected by HIV-1. However, the mechanism involved in HIV-1 internalization into podocytes is not clear. In the present study, we evaluated the occurrence of HIV-1 internalization into conditionally immortalized human podocytes and the mechanism involved. Human podocytes rapidly internalized R5 and X4 HIV-1 primary strains via an endocytosis-dependent pathway, without establishing a productive infection. The HIV-1 internalization was dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN) receptor mediated. The role of DC-SIGN was confirmed by using specific blocking antibodies and transfection with small interfering (si) RNA/DC-SIGN. Since podocyte HIV-1 trafficking was not altered by pH-modulating agents, it appeared that HIV-1 routing occurred through nonacid vesicular compartments. Interestingly, transfection of podocytes with neither siRNA/caveolin-1 nor siRNA/clathrin heavy chain inhibited podocyte viral accumulation. Thus it appears that clathrin-coated vesicles and caveosomes may not be contributing to HIV-1-associated membrane traffic.

References

Jan 1, 1992·Virology·D M Phillips, A S Bourinbaiar
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B M CurtisA J Watson
Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J B KoppP E Klotman
Sep 1, 1991·AIDS Research and Human Retroviruses·G C DouglasB F King
Jan 1, 1991·Journal of Acquired Immune Deficiency Syndromes : JAIDS·A S Bourinbaiar, D M Phillips
Nov 23, 1990·Science·J P MooreQ J Sattentau
Feb 1, 1989·The Journal of Cell Biology·J E Heuser, R G Anderson
Jun 1, 1989·Journal of Virology·J M HarouseF Gonzalez-Scarano
May 1, 1994·Journal of Virology·R Pearce-PrattD M Phillips
Sep 12, 1998·Kidney International·W KrizK V Lemley
Feb 6, 1999·The Journal of the Association of Nurses in AIDS Care : JANAC·C Mallory, B L Fife
Oct 6, 1999·Current Opinion in Structural Biology·K Drickamer
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E J SoilleuxJ Trowsdale
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·S PöhlmannR W Doms
Apr 28, 2001·Cytokine & Growth Factor Reviews·T S Stantchev, C C Broder
Feb 1, 2002·Nature Medicine·Gang MengPhillip D Smith
Feb 22, 2002·Journal of the American Society of Nephrology : JASN·Moin A SaleemPeter Mundel
Mar 26, 2002·Nature Reviews. Immunology·Carl G FigdorGosse J Adema
Jun 11, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tobias Bruno HuberHermann Pavenstädt
Jan 8, 2003·The Journal of Experimental Medicine·Teunis B H GeijtenbeekYvette Van Kooyk
Jan 8, 2003·The Journal of Experimental Medicine·Ludovic TailleuxOlivier Neyrolles
Sep 2, 2003·Nature Reviews. Immunology·Yvette van Kooyk, Teunis B H Geijtenbeek
Nov 6, 2003·Journal of Cell Science·Ben Nichols
Jan 8, 2004·The Journal of Cell Biology·Alessandra CambiCarl G Figdor
Jul 16, 2004·Journal of Virology·Irene S LudwigTeunis B H Geijtenbeek
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diego Serrano-GómezAngel L Corbí
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Madhavan P N NairChiu Bin Hsiao

❮ Previous
Next ❯

Citations

May 31, 2012·The Journal of Biological Chemistry·Guangtao ZhangMing-Ming Zhou
Apr 27, 2011·Current Opinion in HIV and AIDS·John Phair, Frank Palella
Sep 6, 2011·Annual Review of Medicine·Christina M WyattPaul E Klotman
Mar 23, 2015·Experimental and Molecular Pathology·Xiqian LanPravin C Singhal
May 16, 2014·Kidney International·Michael J Ross
Jan 5, 2016·Biomolecular NMR Assignments·Eliška PospíšilováJosef Chmelík
Nov 6, 2013·FEMS Microbiology Reviews·Wander Van BreedamHans J Nauwynck
Oct 12, 2010·Immunology Letters·Pierre Redelinghuys, Gordon D Brown
Oct 9, 2012·Virology·Priyanka SinghPravin C Singhal
Feb 22, 2011·Journal of the American Society of Nephrology : JASN·Mohammad Husain, Pravin C Singhal
Sep 12, 2020·Glycoconjugate Journal·Manuela ValsecchiMassimo Aureli
Nov 5, 2016·Journal of the American Society of Nephrology : JASN·Jinliang LiPatricio E Ray
Oct 7, 2015·Clinical and Experimental Immunology·Minchao CaiWeijie Yuan
Aug 5, 2017·Case Reports in Nephrology·Muhammad Siddique KhurramHong Qu
Nov 25, 2020·AIDS·Kelly HughesMaria Blasi
Oct 13, 2010·Virology·Atanu K KhatuaWaldemar Popik

❮ Previous
Next ❯

Related Concepts

Related Feeds

Caveolins & Signal Transduction

Caveolins are small proteins with a hairpin loop conformation that are located in the plasma membrane of various cell types where they bind cholesterol and interact with receptors essential for several signal transduction pathways. Here is the latest research.