Abstract
The expression and function of DcHsp17.7, a small heat shock protein in carrot (Daucus carota L.), were examined under salt stress, which is an exacerbating environmental condition due to water shortage and irrigation. DcHsp17.7 was constitutively expressed in leaf and stem tissues under normal growth conditions. Upon exposure to 300 mM NaCl, the protein level of DcHsp17.7 increased dramatically in leaf tissue, but did not significantly change in stem tissue. Native-PAGE analysis showed tissue-specific oligomer formation. Under normal growth conditions, DcHsp17.7 was found in an approximately 240 kDa complex in both tissues. However, NaCl treatment induced an additional approximately 160 kDa complex containing DcHsp17.7. This occurred only in leaf tissue, suggesting tissue-specific oligomeric complex formation. To examine the functional mechanism of DcHsp17.7 under stress conditions, the DcHsp17.7 coding gene was introduced into Escherichia coli. Heterologous expression of DcHsp17.7 was induced by isopropyl β-d-1-thiogalactopyranoside treatment. Upon exposure to salinity, protein levels of DcHsp17.7 decreased, and the protein was not detected after 16 hours. Native-PAGE analysis showed that DcHsp17.7 was present in an approxima...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Dec 1, 1996·Cell Stress & Chaperones·L NoverW B Gurley
Mar 30, 1999·Nature Biotechnology·M KasugaK Shinozaki
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M K MalikJ L Zimmerman
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·M KirschnerL Nover
Sep 29, 2001·The Plant Journal : for Cell and Molecular Biology·W SunN Verbruggen
Oct 16, 2001·Cell Stress & Chaperones·K D ScharfE Vierling
Aug 2, 2002·Biochimica Et Biophysica Acta·Weining SunNathalie Verbruggen
Sep 27, 2003·Planta·Wangxia WangArie Altman
Apr 2, 2005·Plant Molecular Biology·Jiahn-Chou GuanChu-Yung Lin
Apr 16, 2005·Current Opinion in Biotechnology·Basia Vinocur, Arie Altman
May 10, 2005·The Plant Cell·Inbal Neta-SharirDavid Weiss
Sep 17, 2005·Trends in Biotechnology·Katherine Denby, Chris Gehring
Oct 6, 2005·Nature Structural & Molecular Biology·Martin HaslbeckJohannes Buchner
Nov 8, 2006·Plant, Cell & Environment·Yeh-Jin Ahn, J Lynn Zimmerman
May 24, 2007·BMC Genomics·William R SwindellAndreas P Weber
Sep 25, 2007·Journal of Experimental Botany·Daisuke OgawaTakumi Nishiuchi
Nov 15, 2007·The Plant Journal : for Cell and Molecular Biology·Franziska SchrammPascal von Koskull-Döring
May 9, 2009·Plant, Cell & Environment·Changhua JiangFeng Ming
Jan 29, 2011·BMC Genomics·Yong-Xiang LinBei-Jiu Cheng
Citations
Apr 27, 2012·Plant Cell Reports·Liping SunXinghong Yang
Feb 19, 2013·PloS One·Yanshi XiaPeiguo Guo
Oct 3, 2013·PloS One·Gianpiero MarconiEmidio Albertini
May 8, 2014·International Journal of Genomics·Bhaskar Gupta, Bingru Huang
Apr 25, 2015·FEBS Letters·Yuanyuan BuShenkui Liu
Apr 12, 2016·Frontiers in Plant Science·Guoqiang FanYongsheng Li
Nov 29, 2012·Journal of Experimental Botany·Yuri Shavrukov
May 11, 2015·Transgenic Research·Punam KumariNeera Bhalla Sarin
Apr 10, 2015·Applied Biochemistry and Biotechnology·Palakolanu Sudhakar ReddyMalireddy K Reddy
Apr 10, 2013·Journal of Proteomics·Jing LiBernard R Glick
Nov 30, 2016·International Journal of Biological Macromolecules·Zeinab TakallooTaghi Moazzenzade
Sep 8, 2019·BMC Plant Biology·Shengmei ZhangDingshi Zha
Nov 19, 2017·Biochemical Genetics·Samia DaldoulThierry Wetzel
Mar 11, 2020·Frontiers in Plant Science·Edward MwandoChengdao Li
Oct 18, 2019·International Journal of Biological Macromolecules·M NagarajuP B Kavi Kishor
Jan 10, 2020·Journal of Proteome Research·Zhou LiYan Peng