DcHsp17.7, a small heat shock protein in carrot, is tissue-specifically expressed under salt stress and confers tolerance to salinity

New Biotechnology
Na-Hyun Song, Yeh-Jin Ahn

Abstract

The expression and function of DcHsp17.7, a small heat shock protein in carrot (Daucus carota L.), were examined under salt stress, which is an exacerbating environmental condition due to water shortage and irrigation. DcHsp17.7 was constitutively expressed in leaf and stem tissues under normal growth conditions. Upon exposure to 300 mM NaCl, the protein level of DcHsp17.7 increased dramatically in leaf tissue, but did not significantly change in stem tissue. Native-PAGE analysis showed tissue-specific oligomer formation. Under normal growth conditions, DcHsp17.7 was found in an approximately 240 kDa complex in both tissues. However, NaCl treatment induced an additional approximately 160 kDa complex containing DcHsp17.7. This occurred only in leaf tissue, suggesting tissue-specific oligomeric complex formation. To examine the functional mechanism of DcHsp17.7 under stress conditions, the DcHsp17.7 coding gene was introduced into Escherichia coli. Heterologous expression of DcHsp17.7 was induced by isopropyl β-d-1-thiogalactopyranoside treatment. Upon exposure to salinity, protein levels of DcHsp17.7 decreased, and the protein was not detected after 16 hours. Native-PAGE analysis showed that DcHsp17.7 was present in an approxima...Continue Reading

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Citations

May 8, 2014·International Journal of Genomics·Bhaskar Gupta, Bingru Huang
Nov 29, 2012·Journal of Experimental Botany·Yuri Shavrukov
Apr 10, 2015·Applied Biochemistry and Biotechnology·Palakolanu Sudhakar ReddyMalireddy K Reddy
Nov 30, 2016·International Journal of Biological Macromolecules·Zeinab TakallooTaghi Moazzenzade

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