DCLK1 immunoreactivity in colorectal neoplasia.

Clinical and Experimental Gastroenterology
Giuseppe GagliardiCharles F Bellows

Abstract

Microtubule-associated doublecortin and CaM kinase-like-1 (DCLK1) is a novel candidate marker for intestinal stem cells. The aim of our study was to assess DCLK1 immunoreactivity in colorectal carcinogenesis and its correlation with prognosis. DCLK1 immunostaining was performed in colorectal tissue from 71 patients, including 18 adenomatous polyps, 40 primary adenocarcinomas, and 14 metastatic lesions. Each case was evaluated by a combined scoring method based on the intensity of staining (score 0-3) and the percentage of tissue staining positive (score 0-3). Immunoexpression for DCLK1 was considered as positive when the combined score was 2-6 and negative with a score of 0-1. Overall, 14/18 (78%) of polyps, 30/40 (75%) of primary adenocarcinomas, and 7/14 (50%) of distant metastases were positive for DCLK1. In adenomatous polyps and primary cancer there was no association between DCLK1 staining score and tumor pathology. However, after curative colorectal cancer resection, patients whose tumor had a high (≥5) combined staining score had increased cancer-specific mortality compared to patients with low (0-4) staining score (hazard ratio 5.89; 95% confidence interval: 1.22-28.47; P = 0.027). We found that DCLK1 is frequently exp...Continue Reading

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Citations

Jun 1, 2014·Cancer Letters·Sripathi M SurebanCourtney W Houchen
Jan 30, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Alireza MirzaeiZahra Madjd
Jul 30, 2016·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Lorenz KadletzClaudia Lill
Jul 28, 2016·Small GTPases·C Benedikt WestphalenTimothy C Wang
Jul 15, 2017·Expert Review of Gastroenterology & Hepatology·Yong Seok KimKi Baik Hahm
Sep 9, 2017·World Journal of Gastroenterology : WJG·Kohei NishioMasaichi Ohira
Jun 21, 2018·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Yuzo HaradaToshiaki Watanabe
Oct 9, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Fatemeh B RassouliMorvarid Saeinasab
Nov 2, 2016·Cancer Biomarkers : Section a of Disease Markers·Alireza MirzaeiGholamreza Tavoosidana
Nov 5, 2019·Frontiers in Oncology·Carmen Muñoz-MaldonadoMichaela Medová
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Apr 18, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shubhashish SarkarPomila Singh
Jul 8, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Moritz MiddelhoffMichael Quante
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Aug 7, 2020·Cell Chemical Biology·Yan LiuKenneth D Westover

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Methods Mentioned

BETA
surgical resection
fluorescence-activated cell sorting
xenograft

Software Mentioned

SPSS

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