DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.

BMC Cancer
Joseph P ConnorNonyem Onujiogu

Abstract

Overcoming platinum resistance is a major obstacle in the treatment of Epithelial Ovarian Cancer (EOC). In our previous work Decoy Receptor 3 (DcR3) was found to be related to platinum resistance. The major objective of this work was to define the cellular interaction of DcR3 with EOC and to explore its effects on platinum responsiveness. We studied cell lines and primary cultures for the expression of and the cells ability to bind DcR3. Cells were cultured with DcR3 and then exposed to platinum. Cell viability was determined by MTT assay. Finally, the cells molecular response to DcR3 was studied using real time RT-PCR based differential expression arrays, standard RT-PCR, and Western blot. High DcR3 in the peritoneal cavity of women with EOC is associated with significantly shorter time to first recurrence after platinum based therapy (p = 0.02). None-malignant cells contribute DcR3 in the peritoneal cavity. The cell lines studied do not secrete DcR3; however they all bind exogenous DcR3 to their surface implying that they can be effected by DcR3 from other sources. DcR3s protein binding partners are minimally expressed or negative, however, all cells expressed the DcR3 binding Heparan Sulfate Proteoglycans (HSPGs) Syndecans-2...Continue Reading

References

Jun 11, 2002·The Journal of Biological Chemistry·Haein ParkEok-Soo Oh
Oct 26, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hiroaki Suzuki, Katsushige Yamashiro
May 13, 2003·International Journal of Cancer. Journal International Du Cancer·Yulian WuJiangping Wu
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Guixiu ShiJiangping Wu
Dec 6, 2003·Journal of Leukocyte Biology·Yung-Chi ChangShie-Liang Hsieh
Dec 31, 2003·Experimental Cell Research·Ming-Jen HsuShie-Liang Hsieh
Mar 27, 2004·The American Journal of Surgical Pathology·Elizabeth MontgomeryAmy Chadburn
May 6, 2004·The Kaohsiung Journal of Medical Sciences·Shiuh-Lin HwangKung-Shing Lee
Aug 7, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·E June DaviesGordon C Jayson
Sep 2, 2004·Journal of Molecular Histology·Innoc HanEok-Soo Oh
Jan 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chia-Ron YangWan-Wan Lin
Mar 9, 2005·Biochemical and Biophysical Research Communications·Jin-Wen HuangNin-Nin Chuang
Mar 31, 2005·Biochemical and Biophysical Research Communications·Ling-Yun ChuNin-Nin Chuang
Apr 13, 2005·Biochemical and Biophysical Research Communications·Jin-Wen HuangNin-Nin Chuang
Sep 28, 2005·International Journal of Cancer. Journal International Du Cancer·Marc D PacificoGeorge D Wilson
Oct 4, 2005·The International Journal of Biochemistry & Cell Biology·Jeffrey J EssnerStephen C Ekker
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yung-Chi ChangShie-Liang Hsieh
Jun 1, 2006·Familial Cancer·William D Foulkes
Sep 26, 2006·Biochemical and Biophysical Research Communications·Jin-Wen Huang, Nin-Nin Chuang
Nov 17, 2007·Blood·Ren-In YouShie-Liang Hsieh
Sep 25, 2008·Neoplasia : an International Journal for Oncology Research·Stephan Macher-GoeppingerWilfried Roth
Oct 29, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David S P TanMartin E Gore
Mar 10, 2009·Journal of Neuroimmunology·André M MuellerAndreas Steinbrecher
Nov 1, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Stephan Macher-GoeppingerSebastian Aulmann
Jan 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shyh-Kuan TaiShie-Liang Hsieh

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Citations

Jun 24, 2015·Biochemical and Biophysical Research Communications·Yi ZhangJian Zhou
Oct 28, 2019·Cancers·Linah F Al-AlemBo R Rueda
Jun 21, 2017·Journal of Biomedical Science·Shie-Liang Hsieh, Wan-Wan Lin
Jun 3, 2021·International Journal of Molecular Sciences·Anett HudákTamás Letoha

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