PMID: 2108732Apr 9, 1990Paper

DDAVP-induced release of von Willebrand factor from endothelial cells in vitro: the effect of plasma and blood cells

Biochimica Et Biophysica Acta
S HashemiP R Ganz

Abstract

The vasopressin analogue 1-deamino-8-D-arginine vasopressin (DDAVP) causes an immediate, transient rise in plasma levels of von Willebrand factor (vWF) after its administration. Although it is recognized that vascular endothelial cells play an essential role in this process, the molecular basis of the response is not understood. We have investigated the phenomenon using human umbilical vein endothelial cells as an in vitro model. When normal individuals were stimulated with DDAVP, plasma from blood samples collected subsequently caused the release of vWF from cultured endothelial cells over a 24 h period (22-46% increase over baseline), compared to control plasma (5-17%). DDAVP added directly to the endothelial cells produced no increase in vWF release. When whole blood was treated in vitro with DDAVP, and the plasma subsequently added to endothelial cells, a significant increase in vWF secretion was found. Peripheral blood mononuclear cells were then tested. In the presence of DDAVP, an increased response occurred. Further fractionation of these cells showed that monocytes were largely responsible, causing an increased vWF release of 162% at 2 h. These observations were reinforced by finding that the supernatants of monocytes ...Continue Reading

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Citations

Oct 1, 1994·Annals of Hematology·S Lethagen
Apr 29, 1993·Regulatory Peptides·J L David
Sep 12, 2000·Haemophilia : the Official Journal of the World Federation of Hemophilia·P M Mannucci
Mar 4, 1993·The New England Journal of Medicine·J A Ware, D D Heistad
Mar 21, 1998·The Journal of Clinical Investigation·J B RosenbergR R Montgomery
Mar 19, 2013·PloS One·Doreen JankeVera Jankowski
Oct 15, 1991·Biochemical and Biophysical Research Communications·P R GanzF Luison
Jun 1, 1992·The American Review of Respiratory Disease·D K JonesN F Voelkel
Nov 5, 1997·The Journal of Surgical Research·F D RubensP R Ganz
Jan 15, 1993·Thrombosis Research·A K DudaniP R Ganz

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