DDB, a putative DNA repair protein, can function as a transcriptional partner of E2F1.

Molecular and Cellular Biology
S A HayesPradip Raychaudhuri

Abstract

The transcription factor E2F1 is believed to be involved in the regulated expression of the DNA replication genes. To gain insights into the transcriptional activation function of E2F1, we looked for proteins in HeLa nuclear extracts that bind to the activation domain of E2F1. Here we show that DDB, a putative DNA repair protein, associates with the activation domain of E2F1. DDB was identified as a heterodimeric protein (48 and 127 kDa) that binds to UV-damaged DNA. We show that the UV-damaged-DNA binding activity from HeLa nuclear extracts can associate with the activation domain of E2F1. Moreover, the 48-kDa subunit of DDB, synthesized in vitro, binds to a fusion protein of E2F1 depending on the C-terminal activation domain. The interaction between DDB and E2F1 can also be detected by coimmunoprecipitation experiments. Immunoprecipitation of an epitope-tagged DDB from cell extracts resulted in the coprecipitation of E2F1. In a reciprocal experiment, immunoprecipitates of E2F1 were found to contain DDB. Fractionation of HeLa nuclear extracts also revealed a significant overlap in the elution profiles of E2F1 and DDB. For instance, DDB, which does not bind to the E2F sites, was enriched in the high-salt fractions containing E2...Continue Reading

References

Feb 1, 1989·Molecular and Cellular Biology·A S YeeJ R Nevins
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·V A BohrP C Hanawalt
Jul 1, 1995·Molecular and Cellular Biology·L N PetersenV A Bohr
Dec 23, 1994·Science·P C Hanawalt
Feb 1, 1995·Molecular and Cellular Biology·X Q QinW G Kaelin
Aug 1, 1994·Genes & Development·B D DynlachtE Harlow
Dec 1, 1994·Genes & Development·K KomachiA D Johnson
Apr 8, 1994·Cell·R DrapkinD Reinberg
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·S KeeneyS Linn
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E K FlemingtonW G Kaelin
Oct 1, 1993·Molecular and Cellular Biology·K HelinA Fattaey
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·W R SellersW G Kaelin

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Citations

Oct 1, 2011·The International Journal of Biochemistry & Cell Biology·Barbara IovineMaria Assunta Bevilacqua
Dec 17, 2009·International Journal of Cancer. Journal International Du Cancer·Bassant M BarakatAltaf A Wani
Jan 28, 2015·Journal of Medical Genetics·Massimo ManginoAbraham Aviv
Dec 12, 2012·Trends in Molecular Medicine·Brigitte M Pützer, David Engelmann
Jan 25, 2005·Molecular Cell·Oleksi Petrenko, Ute M Moll
Jan 31, 2004·Biochemical and Biophysical Research Communications·Taeko InokiHitoshi Endo
Apr 24, 2008·PloS One·Zilal KattanPhilippe Becuwe
Apr 7, 2017·Carcinogenesis·Hongliang LiuUNKNOWN Transdisciplinary Research in Cancer of the Lung (TRICL) Research Team
Oct 31, 2012·International Journal of Molecular Sciences·Nilotpal RoyPradip Raychaudhuri
Aug 19, 2016·Journal of Virology·Woohyun KimWang-Shick Ryu
Dec 10, 1999·The Journal of Biological Chemistry·P ShiyanovP Raychaudhuri
Jul 3, 1999·The Journal of Biological Chemistry·Y FujiwaraS Iwai
Apr 25, 2000·The Journal of Biological Chemistry·W LiuS Linn
May 30, 2003·Genes & Genetic Systems·DongTao FuTsukasa Matsunaga
Jun 17, 2004·The Journal of Biological Chemistry·Abhishek DattaPradip Raychaudhuri
Nov 30, 2000·Molecular and Cellular Biology·R HaraA Sancar
Aug 14, 2020·Cell Cycle·Swarnalatha ManickavinayahamDavid G Johnson
Oct 24, 2001·The Journal of Biological Chemistry·X ChenP Zhou
Sep 12, 2000·Journal of Virology·G Y Lin, R A Lamb
Jul 9, 2005·The Journal of Biological Chemistry·Maria Assunta BevilacquaFiliberto Cimino
Apr 24, 2010·The Journal of Biological Chemistry·Ruifeng GuoDavid G Johnson
Dec 4, 2012·International Journal of Molecular Sciences·Renier Vélez-Cruz, David G Johnson

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