DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells

The Journal of Biological Chemistry
Sonya BaderPeter J M Van Haastert

Abstract

Dictyostelium discoideum cells possess multiple cyclic nucleotide phosphodiesterases that belong either to class I enzymes that are present in all eukaryotes or to the rare beta-lactamase class II. We describe here the identification and characterization of DdPDE4, the third class I enzyme of Dictyostelium. The deduced amino acid sequence predicts that DdPDE4 has a leader sequence, two transmembrane segments, and an extracellular catalytic domain that exhibits a high degree of homology with human cAMP-specific PDE8. Expression of the catalytic domain of DdPDE4 shows that the enzyme is a cAMP-specific phosphodiesterase with a K(m) of 10 microm; cGMP is hydrolyzed at least 100-fold more slowly. The full-length protein is shown to be membrane-bound with catalytic activity exposed to the extracellular medium. Northern blots and activity measurements reveal that expression of DdPDE4 is low during single cell stages and increases at 9 h of starvation, corresponding with mound stage. A function during multicellular development is confirmed by the phenotype of ddpde4(-) knock-out strains, showing normal aggregation but impaired development from the mound stage on. These results demonstrate that DdPDE4 is a unique membrane-bound phospho...Continue Reading

References

May 11, 1992·Proceedings of the National Academy of Sciences of the United States of America·E MeacciV Manganiello
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·G ShaulskyW F Loomis
Jun 10, 1998·Biochemical and Biophysical Research Communications·D A FisherJ B Cheng
Jun 23, 1998·The Journal of Biological Chemistry·S H SoderlingJ A Beavo
Jun 23, 1998·The Journal of Biological Chemistry·D A FisherJ B Cheng
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·S H SoderlingJ A Beavo
Nov 13, 1998·The Journal of Biological Chemistry·H J KimP Schaap
Dec 8, 1998·Molecular Biology of the Cell·M T Laub, W F Loomis
Jan 5, 2002·The Journal of Biological Chemistry·Jeroen Roelofs, Peter J M Van Haastert
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jonathan M GoldbergJanet L Smith
Aug 29, 2002·The EMBO Journal·Leonard BosgraafPeter J M Van Haastert
Nov 14, 2002·Molecular Biology of the Cell·Marcel E MeimaPauline Schaap
Nov 14, 2002·Molecular Biology of the Cell·Leonard BosgraafPeter J M Van Haastert
Feb 8, 2003·The Journal of Biological Chemistry·Marcel E MeimaPauline Schaap
Mar 22, 2003·Developmental Biology·Karin E WeeningPauline Schaap
Sep 4, 2003·Journal of Muscle Research and Cell Motility·Shweta SaranPauline Schaap
Dec 19, 2003·Nucleic Acids Research·Lisa KreppelAlan R Kimmel
Dec 17, 2004·Molecular Biology of the Cell·Douwe M VeltmanPeter J M Van Haastert
May 6, 2005·Nature·L EichingerA Kuspa

❮ Previous
Next ❯

Citations

Aug 30, 2012·BMC Developmental Biology·Pundrik JaiswalRamamurthy Baskar
Jan 25, 2012·BMC Developmental Biology·Pundrik JaiswalRamamurthy Baskar
Mar 30, 2016·Differentiation; Research in Biological Diversity·Tina JubinRasheedunnisa Begum
May 21, 2016·Frontiers in Plant Science·Inonge Gross, Jörg Durner
Jul 1, 2016·Nature Communications·Gernot GlöcknerPauline Schaap
Feb 20, 2007·The Journal of Pharmacology and Experimental Therapeutics·Edwin K JacksonZaichuan Mi
Oct 17, 2006·The Biochemical Journal·Sonya BaderPeter J M Van Haastert
Sep 6, 2006·The Biochemical Journal·Elisa Alvarez-CurtoPauline Schaap

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.