De-methylation of displacement loop of mitochondrial DNA is associated with increased mitochondrial copy number and nicotinamide adenine dinucleotide subunit 2 expression in colorectal cancer

Molecular Medicine Reports
Jinhang GaoShi Feng

Abstract

DNA methylation occurs in the displacement loop (D-loop) region of mammals; however, D-loop regions of certain tumor tissue types were found to be de‑methylated. Whether hypomethylation of the D‑loop region is involved in the regulation of the mitochondrial DNA (mtDNA) copy number and nicotinamide adenine dinucleotide subunit 2 (ND‑2) expressions in colorectal cancer has remained elusive. In the present study, the association between methylation status of the D‑loop region, mtDNA copy number and ND‑2 expression was investigated in 65 colorectal cancer specimens and their corresponding non‑cancerous tissues. In addition, a de‑methylation experiment was performed on the Caco‑2 colorectal cancer cell line by using 5‑aza-2'-deoxycytidine (5‑Aza). The methylation rate of the D‑loop region in all 65 colorectal cancer tissues was markedly reduced when compared with that of their corresponding non‑cancerous tissues (13.8 vs. 81.5%; P<0.05). Furthermore, the methylation rate of the D‑loop region in colorectal cancer tissues was markedly decreased in clinicopathological stages III and IV compared with that in clinicopathological stages I and II (7.1 and 0% vs. 25 and 16%; P<0.05). In addition, the mean relative mtDNA copy number and ND‑2...Continue Reading

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Citations

Jan 17, 2020·DNA and Cell Biology·Ngoc Ngo Yen NguyenYong Hwa Jo
Aug 23, 2020·Antioxidants·Laura Bordoni, Rosita Gabbianelli
Jun 28, 2016·Current Environmental Health Reports·Luca Lambertini, Hyang-Min Byun
Mar 7, 2019·Frontiers in Endocrinology·Fabio Coppedè, Andrea Stoccoro
Oct 31, 2019·Nucleic Acids Research·Vibha PatilZdenko Herceg
Sep 13, 2020·Clinical Epigenetics·Andrea StoccoroFabio Coppedè
Apr 11, 2017·Journal of Cancer Research and Clinical Oncology·Edoardo Errichiello, Tiziana Venesio
Feb 11, 2020·Frontiers in Cell and Developmental Biology·Zhen DongHongjuan Cui
May 1, 2021·International Journal of Molecular Sciences·Andrea Stoccoro, Fabio Coppedè

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