De novo adipocyte differentiation from Pdgfrβ+ preadipocytes protects against pathologic visceral adipose expansion in obesity.

Nature Communications
Mengle ShaoRana K Gupta

Abstract

Pathologic expansion of white adipose tissue (WAT) in obesity is characterized by adipocyte hypertrophy, inflammation, and fibrosis; however, factors triggering this maladaptive remodeling are largely unknown. Here, we test the hypothesis that the potential to recruit new adipocytes from Pdgfrβ+ preadipocytes determines visceral WAT health in obesity. We manipulate levels of Pparg, the master regulator of adipogenesis, in Pdgfrβ+ precursors of adult mice. Increasing the adipogenic capacity of Pdgfrβ+ precursors through Pparg overexpression results in healthy visceral WAT expansion in obesity and adiponectin-dependent improvements in glucose homeostasis. Loss of mural cell Pparg triggers pathologic visceral WAT expansion upon high-fat diet feeding. Moreover, the ability of the TZD class of anti-diabetic drugs to promote healthy visceral WAT remodeling is dependent on mural cell Pparg. These data highlight the protective effects of de novo visceral adipocyte differentiation in these settings, and suggest Pdgfrβ+ adipocyte precursors as targets for therapeutic intervention in diabetes.

References

Oct 1, 1994·Physiological Reviews·A H Kissebah, G R Krakower
Jun 7, 2002·The Journal of Clinical Endocrinology and Metabolism·Yoshinori MiyazakiRalph A DeFronzo
Apr 29, 2006·Obesity·Richard N BergmanMarilyn Ader
Dec 15, 2006·Nature·Jean-Pierre Després, Isabelle Lemieux
Dec 29, 2006·Annals of the New York Academy of Sciences·Istvan SzatmariLaszlo Nagy
Aug 25, 2007·The Journal of Clinical Investigation·Ja-Young KimPhilipp E Scherer
May 6, 2008·Nature·Kirsty L SpaldingPeter Arner
May 8, 2008·Cell Metabolism·Thien T TranC Ronald Kahn
Sep 20, 2008·Science·Wei TangJonathan M Graff
Jul 23, 2009·American Journal of Physiology. Endocrinology and Metabolism·Birgit GustafsonUlf Smith
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Elisa FabbriniSamuel Klein
Jan 9, 2010·Biochimica Et Biophysica Acta·Sam Virtue, Antonio Vidal-Puig
Jan 30, 2010·Obesity·Yuji YamamotoC Ronald Kahn
Jun 10, 2010·Current Opinion in Clinical Nutrition and Metabolic Care·Mi-Jeong LeeSusan K Fried
Jun 24, 2010·American Journal of Physiology. Endocrinology and Metabolism·Nora KlötingMatthias Blüher
Aug 4, 2010·Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery·Olga T HardyMichael P Czech
Jun 3, 2011·The Journal of Clinical Investigation·Kai SunPhilipp E Scherer
Jul 5, 2011·Cell Metabolism·Wei TangJonathan M Graff
Nov 15, 2012·The Journal of Clinical Endocrinology and Metabolism·Kalypso KarastergiouSteven R Smith
Mar 16, 2013·Diabetes Care·Sarah L AppletonUNKNOWN North West Adelaide Health Study Team
May 9, 2013·Nature Medicine·Maryam AhmadianRonald M Evans
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Fenfen WangMitchell A Lazar
Feb 4, 2014·Endocrinología y nutrición : órgano de la Sociedad Española de Endocrinología y Nutrición·Antonio Vidal-Puig

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Citations

Jan 6, 2019·Nature Reviews. Molecular Cell Biology·Alexandra L Ghaben, Philipp E Scherer
May 21, 2019·Endocrinology·Aaron R CoxSean M Hartig
Jan 10, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Laura ButruilleDelphine Eberlé
Feb 7, 2020·The Biochemical Journal·Elizabeth A Rondini, James G Granneman
Jun 17, 2020·The Biochemical Journal·Alice E Pollard, David Carling
Oct 3, 2018·Adipocyte·Elisabeth Kulenkampff, Christian Wolfrum
Feb 10, 2019·Nature Communications·Christian F Guerrero-JuarezMaksim V Plikus
Apr 14, 2019·Biology·Quyen LuongKevin Y Lee
Oct 2, 2019·The Journal of Clinical Investigation·Lavanya Vishvanath, Rana K Gupta
Sep 10, 2019·The Journal of Clinical Investigation·Geneviève MarcelinKarine Clément
Nov 22, 2019·Frontiers in Endocrinology·Talita da Silva Mendes de FariasMaria Isabel Cardoso Alonso-Vale
Nov 7, 2019·International Journal of Molecular Sciences·Michele d'AngeloElisabetta Benedetti
Jul 31, 2019·International Journal of Molecular Sciences·Mohammed El HafidiRoxana Carbó
Aug 8, 2020·Frontiers in Cell and Developmental Biology·Iryna PyrinaAntonios Chatzigeorgiou
Jan 8, 2021·Circulation Research·Shangang ZhaoPhilipp E Scherer
Feb 5, 2021·Cell Stem Cell·Mengle ShaoRana K Gupta
Jan 22, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Liliya V VasilevaMilen I Georgiev
Oct 27, 2020·JCSM Rapid Communications·Gurpreet AroraRodney Infante
Oct 24, 2020·Cells·Hisham F BahmadMohamed Al-Sayegh
Feb 12, 2020·Molecular and Cellular Endocrinology·Zhongyuan WenChanghua Wang
Mar 7, 2021·Nutrients·Vitor Jacó AntracoMaria Isabel Cardoso Alonso-Vale
Mar 18, 2021·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Rachael BatabyalIoannis Koutroulis
Apr 27, 2021·Obesity·Nathan C WinnAlyssa H Hasty
Apr 20, 2021·The Journal of Biological Chemistry·Shuwen QianQi-Qun Tang
Jun 3, 2021·Immunological Reviews·Julia Sbierski-KindAri B Molofsky
Jun 10, 2021·Reviews in Endocrine & Metabolic Disorders·Geneviève Marcelin, Karine Clément
Jul 22, 2021·Nature Reviews. Molecular Cell Biology·David E JamesMorris J Birnbaum
Oct 19, 2021·Molecular Metabolism·Megan StefkovichPatrick Seale
Nov 16, 2021·The Journal of Cell Biology·Vanessa SchmidtThomas E Willnow
Jan 15, 2022·Nature Metabolism·Jessica Cannavino, Rana K Gupta

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Methods Mentioned

BETA
transgenic
transgenics
PCR
ELISA
Flow Cytometry
electrophoresis

Software Mentioned

MuralChaser
GraphPad Prism7
Keyence BZ - X Analyzer
Excel

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