De novo adipose formation in a vascularized engineered construct

Microsurgery
Robert L WaltonLiza Wu

Abstract

While the field of tissue engineering is a burgeoning one, progress with fat engineering has lagged, due in large part to problems associated with nurturing and sustaining this unique tissue in vivo. In a pilot study using an experimental rat model, we induced liponeogenesis with a combination of Matrigel and basic fibroblast growth factor in an fibrovascular scaffold, isolating the construct to a pedicled blood supply (the superficial inferior epigastric vessels) via a silicone housing, creating an engineered three-dimensional adipose tissue construct. Adipose tissue and vascular ingrowth were assessed histologically and followed by serial study at 4-week intervals for 16 weeks. We demonstrated persistence of shaped adipose tissue constructs over time, and postulate that incorporating a vascular supply may enhance the durability of experimentally induced fat constructs and potentially provide a means for microsurgical transfer of the construct.

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Citations

Nov 19, 2003·Clinics in Plastic Surgery·Elisabeth K BeahmCharles W Patrick
Feb 12, 2009·Tissue Engineering. Part a·Jennifer H KangDavid L Kaplan
Jun 12, 2008·Tissue Engineering. Part B, Reviews·Charles W PatrickCindy Frye
Feb 20, 2010·Tissue Engineering. Part B, Reviews·Jennifer H ChoiDavid L Kaplan
May 24, 2007·Plastic and Reconstructive Surgery·Matthew R KaufmanJames P Bradley
Jun 19, 2007·Plastic and Reconstructive Surgery·Andrea Moreira GonzalezIan T Jackson
May 28, 2011·Plastic and Reconstructive Surgery·Juergen H DoldererKeren M Abberton
Sep 13, 2005·In Vitro Cellular & Developmental Biology. Animal·Cynthia A FryeCharles W Patrick
Sep 29, 2007·Annals of Plastic Surgery·Vincenzo VindigniRoberta Cortivo
Oct 27, 2006·Expert Review of Medical Devices·Karsten Hemmrich, Dennis von Heimburg
Sep 16, 2009·Expert Review of Medical Devices·Maria Cristina Tanzi, Silvia Farè
Jun 19, 2008·Vascular Pharmacology·Kurt Ming-Chao LinSubbiah Rajasekaran
Dec 7, 2007·The American Journal of Pathology·John A RophaelGeraldine M Mitchell
May 10, 2008·Journal of Biomedical Materials Research. Part a·L FlynnK A Woodhouse
Oct 4, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Masaki YazawaTatsuo Nakajima
Oct 31, 2013·Journal of Tissue Engineering and Regenerative Medicine·Katharina WittmannTorsten Blunk
Jul 17, 2004·Annual Review of Biomedical Engineering·Charles W Patrick
Apr 2, 2009·Organogenesis·Lauren Flynn, Kimberly A Woodhouse
Apr 17, 2010·Advanced Drug Delivery Reviews·Petra Bauer-KreiselTorsten Blunk
Apr 25, 2017·Tissue Engineering. Part B, Reviews·Luke E VisscherDietmar W Hutmacher

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