De novo assembly and annotation of the salivary gland transcriptome of Rhipicephalus appendiculatus male and female ticks during blood feeding

Ticks and Tick-borne Diseases
Minique H de CastroBen J Mans

Abstract

Tick secretory proteins modulate haemostasis, inflammation and immune responses of the host and are attractive recombinant anti-tick vaccine candidates. Yet, many of the proteins have not been characterised due to the limited sequence availability for ticks and other arthropods for homology-based annotation. To address this limitation, we sequenced the salivary glands of the economically important adult male and female Rhipicephalus appendiculatus ticks during feeding. The quality-filtered Illumina sequencing reads were de novo assembled to generate a R. appendiculatus sialotranscriptome of 21,410 transcripts. A non-redundant set of 12,761 R. appendiculatus proteins was predicted from the transcripts, including 2134 putative secretory and 8237 putative housekeeping proteins. Secretory proteins accounted for most of the expression in the salivary gland transcriptome (63%). Of the secretory protein class, the Glycine-rich superfamily contributed 66% and the Lipocalin family 12% of the transcriptome expression. Differential expression analysis identified 1758 female and 2346 male up-regulated transcripts, suggesting varying blood-feeding mechanisms employed between female and male ticks. The sialotranscriptome assembled in this wo...Continue Reading

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Citations

Feb 13, 2016·Ticks and Tick-borne Diseases·Ben J MansAbdalla A Latif
Oct 18, 2016·Insect Biochemistry and Molecular Biology·Immacolata IovinellaFrancesca Romana Dani
Apr 26, 2017·Frontiers in Cellular and Infection Microbiology·José de la FuenteRyan O M Rego
Aug 6, 2017·The Journal of Biological Chemistry·Jenni HaywardMartin J Stone
Oct 17, 2017·Frontiers in Cellular and Infection Microbiology·Sandra AntunesAna Domingos
Jul 25, 2019·Frontiers in Physiology·Iveta ŠtibrániováMária Kazimírová
Feb 11, 2020·Frontiers in Cellular and Infection Microbiology·Poliana Fernanda GiachettoRenato Andreotti
Jul 13, 2019·Frontiers in Physiology·Jindřich ChmelařMichail Kotsyfakis
Jul 27, 2017·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Gabriel Alves LandulfoDarci Moraes Barros-Battesti
Nov 3, 2020·Parasite Immunology·Larissa Almeida MartinsMichail Kotsyfakis
Feb 6, 2020·Ticks and Tick-borne Diseases·Stephen LuAparecida S Tanaka
Nov 9, 2021·Frontiers in Cellular and Infection Microbiology·Shoumo Bhattacharya, Patricia Anne Nuttall

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