PMID: 9533767Apr 9, 1998Paper

De novo expression of the alpha5beta1-fibronectin receptor in HT29 colon-cancer cells reduces activity of C-SRC. Increase of C-SRC activity by attachment on fibronectin

International Journal of Cancer. Journal International Du Cancer
R SchmidtE D Kreuser

Abstract

Changes in integrin expression during malignant transformation have been observed in many tumors. Colon-carcinoma cells show reduced expression or even loss of the alpha5beta1 integrin compared to normal or adenoma cells. To determine the significance of absent alpha5beta1 integrin signaling, we transfected the cDNA coding for the alpha5 integrin sub-unit into the human colon-carcinoma cell line HT29, which constitutively lacks this subunit but does express the beta1 subunit. We show here that the newly expressed fibronectin receptor alpha5beta1 generates multiple signals, causing marked changes in cytoskeletal arrangements within a few minutes of adhesion to fibronectin. Cells expressing the alpha5beta1 integrin exhibit the formation of actin stress fibers and focal adhesions, as well as the induction of tyrosine phosphorylation of several proteins, within 10 min. We identified the focal adhesion kinase pp125FAK and the cytoskeletal protein paxillin as major phosphorylation substrates in these cells. These proteins remained hypophosphorylated when alpha5-negative control cells were plated on fibronectin. The tyrosine kinase pp60c-src, regarded as central in the regulation of cellular proliferation and constitutively over-expre...Continue Reading

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Citations

Nov 30, 2010·Cell Death and Differentiation·H Sanchez-RuderischS Rosewicz
Nov 7, 2002·Oncogene·T Christopher WindhamGary E Gallick
Jun 22, 2000·International Journal of Cancer. Journal International Du Cancer·K SchrammE D Kreuser
Sep 10, 2020·Journal of Receptor and Signal Transduction Research·Jianan WangChengbin Wang
Jan 8, 2021·OncoTargets and Therapy·Jianbing HouHongjuan Cui

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