De Novo Generation and Characterization of New Zika Virus Isolate Using Sequence Data from a Microcephaly Case

MSphere
Yin Xiang SetohAlexander A Khromykh

Abstract

Zika virus (ZIKV) has recently emerged and is the etiological agent of congenital Zika syndrome (CZS), a spectrum of congenital abnormalities arising from neural tissue infections in utero. Herein, we describe the de novo generation of a new ZIKV isolate, ZIKVNatal, using a modified circular polymerase extension reaction protocol and sequence data obtained from a ZIKV-infected fetus with microcephaly. ZIKVNatal thus has no laboratory passage history and is unequivocally associated with CZS. ZIKVNatal could be used to establish a fetal brain infection model in IFNAR-/- mice (including intrauterine growth restriction) without causing symptomatic infections in dams. ZIKVNatal was also able to be transmitted by Aedes aegypti mosquitoes. ZIKVNatal thus retains key aspects of circulating pathogenic ZIKVs and illustrates a novel methodology for obtaining an authentic functional viral isolate by using data from deep sequencing of infected tissues. IMPORTANCE The major complications of an ongoing Zika virus outbreak in the Americas and Asia are congenital defects caused by the virus's ability to cross the placenta and infect the fetal brain. The ability to generate molecular tools to analyze viral isolates from the current outbreak is e...Continue Reading

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Citations

Nov 3, 2017·Journal of Virology·Tomokazu TamuraYoshiharu Matsuura
Oct 5, 2018·Viruses·Yin Xiang SetohAlexander A Khromykh
Oct 17, 2018·The Journal of General Virology·Lindomar José PenaAntonio Carlos de Freitas
Dec 15, 2017·The Journal of General Virology·Joanna ZmurkoReginald F Clayton
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Jul 7, 2017·Infection & chemotherapy·Sl Ki LimIn Kyu Yoon
Jul 14, 2018·Viruses·Maximilian MünsterRalf Bartenschlager
Jan 30, 2020·Frontiers in Immunology·Alessandra S SchanoskiAndreas Suhrbier
Mar 20, 2019·Nature Microbiology·Yin Xiang SetohAlexander A Khromykh
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Nov 6, 2018·Viruses·Ginés Ávila-PérezLuis Martínez-Sobrido
Jun 22, 2020·Current Opinion in Virology·Camila R Fontes-GarfiasPei-Yong Shi
Sep 28, 2020·Antiviral Research·Ricardo Sanchez-VelazquezMargus Varjak
Mar 16, 2021·Frontiers in Microbiology·Iasmim Silva de MelloMyrna Cristina Bonaldo
Jun 10, 2021·Nature Communications·Alberto A AmarillaAlexander A Khromykh

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Datasets Mentioned

BETA
KU527068

Methods Mentioned

BETA
transfection
PCR

Software Mentioned

Gblocks

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