De novo glutamine synthesis induced by corticosteroids in vivo in rats is secondary to weight loss

Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition
I de BlaauwNicolaas E P Deutz

Abstract

Corticosteroid treatment affects muscle protein and glutamine metabolism. In the present study we aimed to clarify to what extent anorexia, weight loss and corticosteroids determine protein and glutamine metabolism in muscle. The study was performed in Wistar rats (300-350 g, n = 40) given triamcinolone (0.25 mg/kg/day i.m.) treatment (CS group) for 14 days, sham treated free fed (FF group), sham treated pair fed (PF group) and sham treated pair weight (PW group). In vivo protein and glutamine turnover were measured using L-[2,6-3H]phenylalanine and L-[3,4-3H]glurtamine as tracer in a three compartment model across the hindquarter. Corticosteroid treatment decreased total body weight to a greater extent than can be explained by decreased food intake only, justifying the need for pair weight controls. Muscle weight loss was relatively greater in the corticosteroid treated rats than in the pair weight controls indicating specific corticosteroid induced changes in muscle protein metabolism. Pair weight rats increased muscle net protein breakdown rates from -5 +/- 3 nmol x 100 g body weight(-1) x min(-1) to -15 +/- 3 nmol x 100 g body weight(-1) x min(-1) (P < 0.05 vs FF). In the corticosteroid treated rats net protein breakdown ra...Continue Reading

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Citations

Feb 10, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·H DongK J Jiang
Sep 20, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Theodorus B M HakvoortWouter H Lamers
Dec 29, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ronan ThibaultDominique Darmaun
Jun 22, 2017·Phytotherapy Research : PTR·Vivian Petersen WagnerManoela Domingues Martins

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