De novo intestine-specific transcriptome of the brown planthopper Nilaparvata lugens revealed potential functions in digestion, detoxification and immune response

Genomics
Yan-Yuan BaoChuan-Xi Zhang

Abstract

The brown planthopper (Nilaparvata lugens, BPH) is the most serious rice plant pests in Asia. In this study, we performed transcriptome-wide analysis on BPH intestine. We obtained more than 26 million sequencing reads that were then assembled into 53,553 unigenes with a mean size of 388 bp. Based on similarity search with the nucleotide sequences available at NCBI, BPH intestine-specific transcriptome analysis identified 21,405 sequences. Assembled sequences were annotated with gene description, gene ontology and clusters of orthologous group terms. The digestion-, defense- and xenobiotic metabolism-related genes were abundantly detected in the transcripts from BPH intestine. Many novel genes including 33 digestion-related genes, 25 immune responsive genes and 27 detoxification-related genes are first reported here. We investigated the gene expression patterns at the transcript levels in different tissues by quantitative real-time PCR analysis, which revealed that some genes had intestine-specific expression, implicating their potential significance for BPH management.

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Citations

Dec 4, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Jirapong JairinHideshi Yasui
Nov 13, 2012·Expert Opinion on Drug Discovery·Nagasuma Chandra, Jyothi Padiadpu
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Aug 20, 2016·BMC Genomics·Bing YuChuan-Xi Zhang
Sep 10, 2020·Frontiers in Physiology·Chenchen ZhaoJinjie Cui
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May 5, 2016·Journal of Proteome Research·Hai-Jian HuangYan-Yuan Bao

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