De novo production of resveratrol from glucose or ethanol by engineered Saccharomyces cerevisiae

Metabolic Engineering
Mingji LiJens Nielsen

Abstract

Resveratrol is a natural antioxidant compound, used as food supplement and cosmetic ingredient. Microbial production of resveratrol has until now been achieved by supplementation of expensive substrates, p-coumaric acid or aromatic amino acids. Here we engineered the yeast Saccharomyces cerevisiae to produce resveratrol directly from glucose or ethanol via tyrosine intermediate. First we introduced the biosynthetic pathway, consisting of tyrosine ammonia-lyase from Herpetosiphon aurantiacus, 4-coumaryl-CoA ligase from Arabidopsis thaliana and resveratrol synthase from Vitis vinifera, and obtained 2.73 ± 0.05 mg L(-1) resveratrol from glucose. Then we over-expressed feedback-insensitive alleles of ARO4 encoding 3-deoxy-D-arabino-heptulosonate-7-phosphate and ARO7 encoding chorismate mutase, resulting in production of 4.85 ± 0.31 mg L(-1) resveratrol from glucose as the sole carbon source. Next we improved the supply of the precursor malonyl-CoA by over-expressing a post-translational de-regulated version of the acetyl-CoA carboxylase encoding gene ACC1; this strategy further increased resveratrol production to 6.39 ± 0.03 mg L(-1). Subsequently, we improved the strain by performing multiple-integration of pathway genes resulting...Continue Reading

References

Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·S OstergaardJ Nielsen
Sep 10, 2005·Applied and Environmental Microbiology·Yajun YanMattheos A G Koffas
Aug 4, 2006·Applied and Environmental Microbiology·Jules BeekwilderArnaud Bovy
Sep 5, 2006·Biochimica Et Biophysica Acta·Oksana TehlivetsSepp D Kohlwein
Oct 5, 2006·Journal of the American Chemical Society·Yansheng ZhangOliver Yu
Oct 5, 2006·Medicinal Research Reviews·Gopal SrinivasPriya Srinivas
Mar 30, 2010·Applied and Environmental Microbiology·Tobias SydorEckhard Boles
Nov 22, 2011·Journal of Biotechnology·Yechun Wang, Oliver Yu
Jan 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Patrick J WestfallChris J Paddon
Jun 2, 2012·Journal of Biomedicine & Biotechnology·Philippe JeandetEric Courot
Aug 14, 2012·Enzyme and Microbial Technology·So-Yeon ShinJin-Ho Seo
Nov 21, 2012·Metabolic Engineering·Kathleen A CurranHal S Alper
Dec 12, 2012·Microbial Cell Factories·Frank KoopmanJean-Marc Daran
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lei Shi, Benjamin P Tu
Jul 17, 2013·Annals of the New York Academy of Sciences·Morten Møller PoulsenSteen Bønløkke Pedersen
Oct 2, 2013·Molecular and Cellular Biology·Man ZhangAles Vancura
Mar 29, 2014·Biotechnology Journal·Irina Borodina, Jens Nielsen
Sep 3, 2014·Biotechnology Letters·Yan-Zhen MeiChuan-Chao Dai
May 29, 2015·Microbial Cell Factories·Nicholas D GoldVincent J J Martin

❮ Previous
Next ❯

Citations

Mar 8, 2016·Plant Biotechnology Journal·Ascensión Martínez-MárquezRoque Bru-Martínez
Mar 12, 2016·Cell·Jens Nielsen, Jay D Keasling
Jul 14, 2016·Applied Microbiology and Biotechnology·Yao LuMingliang Jin
Aug 19, 2016·Critical Reviews in Biotechnology·Isak S Pretorius
Sep 30, 2016·Microbial Cell Factories·José M Camacho-ZaragozaGuillermo Gosset
Nov 7, 2016·Metabolic Engineering·Zhenya ChenQipeng Yuan
Nov 12, 2016·Scientific Reports·Mingji LiJens Nielsen
Jan 24, 2017·Metabolic Engineering·Dandan XiongShuang-Yan Tang
Mar 30, 2017·Applied Microbiology and Biotechnology·Manuela GottardiEckhard Boles
May 13, 2017·Chemical Reviews·Fabio ParmeggianiNicholas J Turner
Mar 17, 2017·Annual Review of Biochemistry·Jens Nielsen
Jun 25, 2017·Trends in Biotechnology·Shuhei Noda, Akihiko Kondo
Jul 29, 2018·World Journal of Microbiology & Biotechnology·A BragaN Faria
Jun 20, 2018·Biotechnology Journal·Mingfeng CaoZengyi Shao
Jun 24, 2018·Applied and Environmental Microbiology·Michael E PyneVincent J J Martin
Apr 27, 2018·The Journal of General and Applied Microbiology·Jiwei MaoMingqiang Qiao
Mar 9, 2019·PLoS Computational Biology·Fangzhou ShenZhuo Wang
May 16, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Laure-Anne PeyratNikolas Fokialakis
Mar 30, 2019·Biotechnology Journal·Jens Nielsen
Jul 18, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samir Bahadur ThapaJae Kyung Sohng
Mar 17, 2020·Annual Review of Plant Biology·Joseph R Jacobowitz, Jing-Ke Weng
Aug 23, 2020·Genes·Mikhail A Eldarov, Andrey V Mardanov
Sep 8, 2019·Nature Communications·Christian Bille Jendresen, Alex Toftgaard Nielsen
Mar 23, 2017·Journal of Industrial Microbiology & Biotechnology·Junjun WuMingsheng Dong
May 22, 2017·Applied Microbiology and Biotechnology·Jens NielsenIvan Mijakovic
Sep 29, 2017·Critical Reviews in Biotechnology·Thomas DecoeneMarjan De Mey
Jul 5, 2018·Microbial Cell Factories·Mark LevissonJules Beekwilder
Jun 14, 2018·Natural Product Reports·Benjamin J KotopkaChristina D Smolke
Feb 28, 2019·Microbial Cell Factories·Damla HuccetogullariSang Yup Lee
Feb 25, 2019·Applied Microbiology and Biotechnology·Anil ShresthaJae Kyung Sohng
Apr 26, 2019·Applied Microbiology and Biotechnology·Antonio G CordenteChristopher D Curtin
Apr 10, 2020·Applied Microbiology and Biotechnology·Endang Rahmat, Youngmin Kang
Mar 24, 2018·Nature Chemistry·Sijin LiChristina D Smolke
May 26, 2018·World Journal of Microbiology & Biotechnology·Nguyen Huy ThuanSailesh Malla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.