De novo protein biosynthesis responses to water stresses in wood frogs: freeze-thaw and dehydration-rehydration

Cryobiology
K B StoreyT A Chruchill

Abstract

Protein biosynthesis responses occurring during the postthaw (after 12 h freezing at -1.4 degrees C), dehydration (to 27 or 40% of total body water lost), or rehydration (after the loss of 40% of body water) were monitored in tissues of spring-collected wood frogs (Rana sylvatica) after intraperitoneal injection of 35S-labeled methionine + cysteine. All six organs tested accumulated radiolabeled amino acids and organs of both thawing and rehydrating frogs held at 3-5 degrees C showed a linear increase in amino acid incorporation into the acid-precipitable protein fraction over time. By contrast, dehydrating animals showed little or no increment in protein bound radioactivity over the course of the stress, a result that may be indicative of metabolic suppression in organs when dehydration became severe. Isoelectrofocusing (IEF) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used to characterize the proteins synthesized by liver under each experimental state. IEF revealed both new peaks of 35S-labeled proteins and enhanced labeling of others in extracts from experimental animals, compared with controls. In particular, new synthesis of proteins with isoelectric points of about 6.0 was prominent and l...Continue Reading

References

Jan 1, 1992·Annual Review of Physiology·K B Storey, J M Storey
Jul 1, 1992·General and Comparative Endocrinology·T P Mommsen, K B Storey
Jan 2, 1992·Nature·M J Gething, J Sambrook
Jan 1, 1988·Physiological Reviews·K B Storey, J M Storey
Jan 1, 1971·Analytical Biochemistry·N Catsimpoolas, J Wang
Jun 1, 1983·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·C A Ketola-Pirie, B G Atkinson
Jan 1, 1994·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·T A Churchill, K B Storey
Mar 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J P CostanzoJ R Layne
Jan 1, 1993·Critical Reviews in Toxicology·B M Sanders
May 1, 1997·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·C P Holden, K B Storey

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Citations

Feb 23, 1999·Clinical and Experimental Pharmacology & Physiology·K B Storey
Nov 13, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Steven A Higgins, David L Swanson
Jan 29, 2002·Cryobiology·K J Cowan, K B Storey
Apr 9, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Armelle AnsartPhilippe Vernon

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