De novo purine synthesis in Arabidopsis thaliana. II. The PUR7 gene encoding 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole synthetase is expressed in rapidly dividing tissues

Plant Physiology
J F SenecoffR B Meagher

Abstract

The small genome size and excellent genetics of Arabidopsis, as well as the ease with which it is transformed, make it a superb candidate for molecular genetic studies of the purine biosynthetic pathway. Herein we report the isolation, physical characterization, and dissection of the expression patterns of the single gene encoding 5'-phosphoribosyl-4-(N-succinocarboxamide)-5-aminoimidazole synthetase. This enzyme, encoded by the PUR7 gene, catalyzes aspartate addition at the alpha-amino group to the growing purine backbone. The expression of the PUR7 as directed by the 5' region, containing the promoter, mRNA leader, and leader intron, was examined in Arabidopsis using a transgenic reporter system. Our analysis demonstrates that the highest level of purine biosynthesis occurs in mitotically active tissues of the plant. Furthermore, purine biosynthesis appears to be under developmental and hormonal regulation. Inhibition of purine biosynthesis using substrate analogs results in arrested plant development and induction of purine gene expression. Purine nucleotides and their derivatives provide multiple cofactors for a variety of metabolic processes. Our findings begin to identify some of the regulatory mechanisms that affect the ...Continue Reading

References

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Citations

Jan 1, 2002·The Arabidopsis Book·Barbara A Moffatt, Hiroshi Ashihara
Mar 20, 2003·Plant Physiology·Danica Erin GogginPenelope Mary Collina Smith
May 4, 2006·Annual Review of Plant Biology·Rita ZrennerRalf Boldt
Jan 23, 2014·Journal of Experimental Botany·Tengbo HuangVivian F Irish
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·Z VaghchhipawalaS Mackenzie
Mar 26, 2003·Physiologia Plantarum·Ralf Boldt, Rita Zrenner
Jun 20, 2008·The Plant Journal : for Cell and Molecular Biology·Simon KirchbergerH Ekkehard Neuhaus
Jun 23, 2015·The Plant Journal : for Cell and Molecular Biology·Niu YuNam-Hai Chua
Jul 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Vasilios M E Andriotis, Alison M Smith
Oct 24, 2019·Journal of Experimental Botany·Carlos Hernán Barrera-RojasFabio Tebaldi Silveira Nogueira
Jan 9, 2019·Frontiers in Plant Science·Jay P MauryaMariano Perales
Sep 6, 2020·BMC Plant Biology·Magda GrabsztunowiczPaula Mulo
Dec 9, 2003·Journal of Plant Physiology·Claudio StasollaHiroshi Ashihara

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