De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase

Molecular and Cellular Biology
Yoshiko MaidaKenkichi Masutomi

Abstract

RNA-dependent RNA polymerase (RdRP) plays key roles in RNA silencing to generate double-stranded RNAs. In model organisms, such as Caenorhabditis elegans and Neurospora crassa, two types of small interfering RNAs (siRNAs), primary siRNAs and secondary siRNAs, are expressed; RdRP produces secondary siRNAs de novo, without using either Dicer or primers, while primary siRNAs are processed by Dicer. We reported that human telomerase reverse transcriptase (TERT) has RdRP activity and produces endogenous siRNAs in a Dicer-dependent manner. However, de novo synthesis of siRNAs by human TERT has not been elucidated. Here we show that the TERT RdRP generates short RNAs that are complementary to template RNAs and have 5'-triphosphorylated ends, which indicates de novo synthesis of the RNAs. In addition, we confirmed short RNA synthesis by TERT in several human carcinoma cell lines and found that TERT protein levels are positively correlated with RdRP activity.

References

Sep 5, 2001·Virology·C C KaoD J Ecker
Apr 24, 2004·The Journal of General Virology·Alberdina A van DijkDennis H Bamford
Jul 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Neal F LueSiyang Leng
Dec 13, 2006·Science·Titia SijenRonald H A Plasterk
Nov 16, 2007·Nature·Elisabeth LehmannPatrick Cramer
Jan 23, 2009·Nature·Haruhiko Siomi, Mikiko C Siomi
Aug 25, 2009·Nature·Yoshiko MaidaKenkichi Masutomi
Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Naoko OkamotoKenkichi Masutomi
Dec 7, 2011·Antimicrobial Agents and Chemotherapy·Guanghui YiC Cheng Kao
Aug 11, 2012·Mini Reviews in Medicinal Chemistry·Yoshiyuki MizushinaHiromi Yoshida
Jan 26, 2013·Science·Franklin W HuangLevi A Garraway
Feb 20, 2014·Molecular and Cellular Biology·Yoshiko MaidaKenkichi Masutomi
Nov 5, 2014·Nature Genetics·Yasushi TotokiTatsuhiro Shibata
Oct 16, 2015·Nature·Martin PeiferMatthias Fischer

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Citations

Jan 5, 2011·PloS One·Hélène PelczarYannick Andéol
Mar 12, 2020·Journal of Molecular Evolution·Ľubomír Tomáška, Jozef Nosek
Feb 1, 2020·Current Topics in Medicinal Chemistry·Connor Ah Thompson, Judy My Wong
Sep 24, 2019·Current Aging Science·Joan Smith-Sonneborn
Mar 28, 2020·Nature Communications·Mami YasukawaKenkichi Masutomi
Oct 11, 2019·International Journal of Molecular Sciences·Pauline Billard, Delphine A Poncet
Sep 1, 2020·Reviews in the Neurosciences·Antonio GiudittaAdolfo Sadile
Oct 27, 2020·Frontiers in Genetics·Wen-Juan NiXiao-Min Leng
May 25, 2021·Frontiers in Molecular Biosciences·Sneha Paturi, Mandar V Deshmukh
Jul 20, 2021·Acta Histochemica Et Cytochemica·Hiromi OnizukaNoriyuki Shibata
Aug 6, 2021·Frontiers in Oncology·Yanni MaHanlin Zeng
Sep 4, 2021·Cancer Medicine·Fumiko ArakawaKoichi Ohshima

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