De novo sequencing and analysis of Lophophora williamsii transcriptome, and searching for putative genes involved in mescaline biosynthesis

BMC Genomics
Enrique Ibarra-LacletteLuis Herrera-Estrella

Abstract

Lophophora williamsii (commonly named peyote) is a small, spineless cactus with psychoactive alkaloids, particularly mescaline. Peyote utilizes crassulacean acid metabolism (CAM), an alternative form of photosynthesis that exists in succulents such as cacti and other desert plants. Therefore, its transcriptome can be considered an important resource for future research focused on understanding how these plants make more efficient use of water in marginal environments and also for research focused on better understanding of the overall mechanisms leading to production of plant natural products and secondary metabolites. In this study, two cDNA libraries were generated from L. williamsii. These libraries, representing buttons (tops of stems) and roots were sequenced using different sequencing platforms (GS-FLX, GS-Junior and PGM, respectively). A total of 5,541,550 raw reads were generated, which were assembled into 63,704 unigenes with an average length of 564.04 bp. A total of 25,149 unigenes (62.19 %) was annotated using public databases. 681 unigenes were found to be differentially expressed when comparing the two libraries, where 400 were preferentially expressed in buttons and 281 in roots. Some of the major alkaloids, incl...Continue Reading

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Citations

Apr 16, 2016·Current Opinion in Plant Biology·James HartwellSusanna F Boxall
Apr 29, 2021·Chemical Society Reviews·Cooper S JamiesonJohn M Billingsley
Jul 16, 2021·Journal of Natural Products·Camilla B ChanJesper L Kristensen
May 31, 2018·ACS Chemical Neuroscience·Bruce K Cassels, Patricio Sáez-Briones

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Datasets Mentioned

BETA
PRJNA261064

Methods Mentioned

BETA
454 Genome Sequencer
RNA-seq
PCR
reverse transcription PCR
Assay
chip
chips

Software Mentioned

Muscle
WEGO
MIRA assembler
SeaView
theorgan
FASTX
CAP3
SEMARNAT
SeqClean
BLAST

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