De novo syntheses of Marburg virus antigens from adenovirus vectors induce potent humoral and cellular immune responses

Vaccine
Danher WangJohn Y Dong

Abstract

Marburg virus (MARV) is an African filovirus that causes a deadly hemorrhagic fever in humans, with up to 90% mortality. Currently, there are no MARV vaccines or therapies approved for human use. We hypothesized that developing a vaccine that induces a de novo synthesis of MARV antigens in vivo will lead to strong induction of both a humoral and cell-mediated immune response against MARV. Here, we develop and characterize three novel gene-based vaccine candidates which express the viral glycoprotein (GP) from either the Ci67, Ravn or Musoke strain of MARV. Immunization of mice with complex adenovirus (Ad)-based vaccine candidates (cAdVax vaccines), led to efficient production of both antibodies and cytotoxic T lymphocytes (CTL) specific to Musoke strain GP and Ci67 strain GP, respectively. Antibody responses were also shown to be cross-reactive across the MARV strains, but not cross-reactive to Ebola virus, a related filovirus. Additionally, three 1 x 10(8)pfu doses of vaccine vector were demonstrated to be safe in mice, as this did not lead to any detectable toxicity in liver or spleen. These promising results indicate that a cAdVax-based vaccine could be effective for induction of both humoral and cell-mediated immune respons...Continue Reading

Citations

Aug 29, 2009·Virology Journal·Warren V KalinaSina Bavari
Dec 4, 2012·Viruses·Kristina BrauburgerJudith Olejnik
Dec 8, 2007·Molecular Aspects of Medicine·Paolo AscenziGiuseppe Ippolito
Nov 22, 2012·Viruses·Steven B BradfuteMike Bray
Dec 29, 2006·Clinical and Vaccine Immunology : CVI·David H HolmanJohn Y Dong
Feb 26, 2010·Clinical and Vaccine Immunology : CVI·William D PrattJohn Y Dong
Jan 25, 2008·Clinical and Vaccine Immunology : CVI·Dana L SwensonWilliam D Pratt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.