PMID: 3756223Oct 1, 1986Paper

De novo synthesis of glutathione in human fibroblasts during in vitro ageing and in some metabolic diseases as measured by a flow cytometric method

Biochimica Et Biophysica Acta
M PootJ F Jongkind

Abstract

A flow cytometric method to determine cellular GSH contents has been developed. This method is fast and simple and enables the determination of GSH contents in intact cells. Results obtained with the new method correlate well with the results obtained by a specific biochemical assay for GSH (r = 0.9984; n = 7). The method has been used to determine GSH recovery rates in cultured fibroblasts from healthy subjects and from patients with Werner's syndrome, Spielmeyer-Vogt syndrome and Fanconi's anemia. No obvious differences in GSH recovery rates were observed. GSH recovery rates were also not affected after in vitro ageing. Experiments with cells deficient in GSH synthetase revealed that the observed GSH recovery is exclusively due to de novo synthesis.

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N S KosowerH M Ranney
Oct 31, 1984·Mechanisms of Ageing and Development·M PootJ F Jongkind

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Citations

Apr 1, 1988·Mechanisms of Ageing and Development·M PootJ F Jongkind
Jan 1, 1989·Free Radical Biology & Medicine·J F JongkindR G Baggen
Mar 1, 1991·Mutation Research·M Poot
Feb 1, 1990·International Journal of Andrology·P J Den BoerJ A Grootegoed
Jul 30, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Moon-Moo Kim, Se-Kwon Kim
May 19, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chang-Suk KongYoungwan Seo
Feb 14, 2012·International Journal of Biological Macromolecules·Dai-Nghiep NgoSe-Kwon Kim
Jan 1, 1990·Journal of Clinical Laboratory Analysis·R B ScottP S Swerdlow
Sep 5, 2008·Current Protocols in Cytometry·M Poot
Jan 4, 2012·Carbohydrate Polymers·Mahinda SenevirathneJae-Young Je

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