De novo synthesis of Legionella pneumophila antigens during intracellular growth in phagocytic cells.

Infection and Immunity
M SusaR Marre

Abstract

Legionella pneumophilia is a gram-negative rod which is able to multiply within phagocytic cells. The process of phagocytosis leads to a rapid environmental change that might require a coordinate regulation of gene expression to ensure intracellular survival. Since there is little information on up- and downregulation of genes during the early phases of phagocytosis, we radiolabeled intracellular L. pneumophila at different times after phagocytosis by macrophages of the Mono Mac 6 cell line and immunoprecipitated antigens with antilegionella sera or monoclonal antibodies. We could identify two antigens which were upregulated, one of which was the Mip protein, three antigens which were downregulated, and three antigens which were not detectable in extracellularly grown L. pneumophila. The Mip protein was stained most intensively 4 to 8 h after intracellular infection, suggesting that it is needed during intracellular multiplication rather than initiation of infection. A 44-kDa antigen which was not detectable during extracellular growth was most prominent from 2 to 4 h postinfection when Mono Mac 6 cells were used as phagocytic cells. The 44-kDa antigen was also expressed during growth with Acanthamoeba castelanii, MRC-5, and U9...Continue Reading

References

Apr 1, 1979·Annals of Internal Medicine·G K MorrisD C Mackel
Jan 1, 1979·Epidemiologic Reviews·C V Broome, D W Fraser
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A MarraH A Shuman
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·N P Cianciotto, B S Fields
Feb 1, 1991·Journal of Clinical Microbiology·I SteinmetzD Bitter-Suermann
Jul 1, 1990·The Journal of Infectious Diseases·N P CianciottoN C Engleberg
Jan 1, 1988·Clinical Microbiology Reviews·W C Winn
Mar 15, 1988·International Journal of Cancer. Journal International Du Cancer·H W Ziegler-HeitbrockG Riethmüller
Nov 1, 1987·The Journal of Experimental Medicine·N R Payne, M A Horwitz
Jul 1, 1987·Microbial Pathogenesis·L A Dreyfus
Sep 1, 1980·The Journal of Clinical Investigation·M A Horwitz, S C Silverstein
Feb 14, 1980·The New England Journal of Medicine·T J DonderoW Schaffner
Mar 1, 1995·Clinical and Diagnostic Laboratory Immunology·J H HelbigJ Hacker
Apr 1, 1993·Infection and Immunity·Y Abu KwaikN C Engleberg

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Citations

Feb 12, 2014·Parasitology·Naveed Ahmed Khan, Ruqaiyyah Siddiqui
Nov 26, 2002·Journal of Bacteriology·Gary Faulkner, Rafael A Garduño
Apr 15, 2003·Clinical Microbiology Reviews·Francine Marciano-Cabral, Guy Cabral
Nov 9, 2000·The Journal of Experimental Medicine·S Sturgill-Koszycki, M S Swanson
Feb 11, 1998·Journal of Bacteriology·R A GarduñoP S Hoffman
Dec 22, 1999·Microbes and Infection·O S Harb, Y Abu Kwaik
Nov 5, 1999·Clinical and Diagnostic Laboratory Immunology·A EssigR Marre

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