PMID: 3772152Nov 1, 1986Paper

De novo synthesis of lysozyme by human epidermal cells

The Journal of Investigative Dermatology
V L ChenG P Martinelli

Abstract

The presence of lysozyme in human epidermis was determined in extracts from the surface of human skin and in sonicates of human epidermal cell preparations with the use of a bacteriolytic assay employing Micrococcus lysodeikticus cell walls as substrate. Preincubation of the extracts with the Fab portion of the IgG fraction of an antiserum to human lysozyme abolished the lytic activity of the extracts showing the specificity of the assay. De novo synthesis of lysozyme by human epidermal cells was demonstrated by radiolabeling studies. Human epidermal cells cultured in serum-free medium and pulsed with [3H]leucine for 24 h were sonicated and fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A major band at Mr 14,500 exhibited both immunoreactivity with a rabbit antihuman lysozyme antibody and radioactivity. Gel filtration of the cell sonicate revealed bacteriolytic activity in the fractions containing radioactive and immunoreactive proteins. These findings suggest that lysozyme is newly synthesized by human epidermal cells.

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Citations

Apr 1, 1992·International Journal of Cosmetic Science·J P Forestier
Oct 31, 2012·Angewandte Chemie·Michael WoodClaude Roux
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·J H YoonP Nettesheim
Apr 4, 2008·Molecular Medicine·Lars SteinstraesserTobias Hirsch
Dec 18, 2013·Future Microbiology·Rosanna CoatesMalcolm J Horsburgh
Jan 24, 2018·Toxicon : Official Journal of the International Society on Toxinology·Ophelie PierreRaphaele Le Garrec
Feb 24, 1998·Clinics in Dermatology·J H Cove, E A Eady
Apr 10, 2007·Burns : Journal of the International Society for Burn Injuries·C S SobralL M Ferreira

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