De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments

Nature Communications
Matthew J LeeMartin J Warren

Abstract

Bacterial microcompartments, BMCs, are proteinaceous organelles that encase a specific metabolic pathway within a semi-permeable protein shell. Short encapsulation peptides can direct cargo proteins to the lumen of the compartments. However, the fusion of such peptides to non-native proteins does not guarantee encapsulation and often causes aggregation. Here, we report an approach for targeting recombinant proteins to BMCs that utilizes specific de novo coiled-coil protein-protein interactions. Attachment of one coiled-coil module to PduA (a component of the BMC shell) allows targeting of a fluorescent protein fused to a cognate coiled-coil partner. This interaction takes place on the outer surface of the BMC. The redesign of PduA to generate an N-terminus on the luminal side of the BMC results in intact compartments to which proteins can still be targeted via the designed coiled-coil system. This study provides a strategy to display proteins on the surface or within the lumen of the BMCs.

References

Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Aug 6, 2005·Science·Cheryl A KerfeldTodd O Yeates
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B A CunninghamG M Edelman
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Guillaume G B TcherkezT John Andrews
Mar 12, 2008·The Journal of Biological Chemistry·Joshua B ParsonsMichael B Prentice
Aug 6, 2008·Nature Reviews. Microbiology·Todd O YeatesJessup M Shively
Sep 13, 2008·Structure·Christopher S CrowleyTodd O Yeates
Oct 22, 2008·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Shouqiang ChengThomas A Bobik
Aug 4, 2009·Nature Biotechnology·John E DueberJay D Keasling
Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chenguang FanThomas A Bobik
Sep 10, 2010·Annual Review of Microbiology·Cheryl A KerfeldGordon C Cannon
Sep 28, 2010·The Journal of Biological Chemistry·Christopher S CrowleyTodd O Yeates
Sep 29, 2011·Metabolic Engineering·Hanson LeeJohn E Dueber
Oct 25, 2011·Nucleic Acids Research·Robert J ConradoMatthew P DeLisa
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chenguang FanThomas A Bobik
Sep 18, 2012·Journal of Biotechnology·Stefanie FrankMartin J Warren
Nov 16, 2012·Acta Crystallographica. Section D, Biological Crystallography·Allan PangRichard W Pickersgill
Mar 5, 2013·Protein Science : a Publication of the Protein Society·Nicole M WheatleyTodd O Yeates
Apr 13, 2013·Science·Jordan M FletcherDerek N Woolfson
May 31, 2014·The Journal of Biological Chemistry·Allan PangRichard W Pickersgill
Oct 24, 2014·PLoS Computational Biology·Seth D AxenCheryl A Kerfeld
May 8, 2015·Nature Protocols·Lawrence A KelleyMichael J E Sternberg
Nov 16, 2016·Scientific Reports·Matthias J MayerMartin J Warren
Feb 28, 2017·Protein Science : a Publication of the Protein Society·Christopher M JakobsonDanielle Tullman-Ercek
Dec 12, 2017·Nature Chemical Biology·Matthew J LeeMartin J Warren

❮ Previous
Next ❯

Citations

Mar 10, 2020·PloS One·Nolan W KennedyDanielle Tullman-Ercek
Jan 22, 2020·Nature Communications·Gints KalninsKaspars Tars
Jun 27, 2019·Biochemical Society Transactions·Sara Planamente, Stefanie Frank
Nov 24, 2020·Journal of Molecular Biology·Nolan W KennedyDanielle Tullman-Ercek
Jan 16, 2021·Trends in Biochemical Sciences·Andrew M StewartThomas A Bobik
Jun 20, 2020·Current Opinion in Biotechnology·Cheryl A Kerfeld, Markus Sutter
Jan 25, 2020·ACS Synthetic Biology·Caitlin L EdgellDerek N Woolfson
May 11, 2019·Metabolic Engineering·Bryan FerlezCheryl A Kerfeld
Mar 30, 2021·PloS One·Jessica M OchoaTodd O Yeates
Oct 9, 2020·EcoSal Plus·Katie L StewartThomas A Bobik
Jun 15, 2021·Current Opinion in Microbiology·Nolan W KennedyDanielle Tullman-Ercek
Aug 3, 2021·Current Opinion in Microbiology·Lu-Ning LiuJing Yang
May 7, 2019·ACS Synthetic Biology·Abigail J SmithNigel J Savery
Nov 22, 2019·Nano Letters·Eric J YoungDaniel C Ducat
Jan 15, 2022·The Journal of Physical Chemistry. B·Ivan R SasselliSamuel I Stupp

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
confocal microscopy
X-ray
fluorescence microscopy

Software Mentioned

IMOD

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Chenguang FanThomas A Bobik
Current Opinion in Biotechnology
Jefferson S Plegaria, Cheryl A Kerfeld
Communicative & Integrative Biology
Clément AussignarguesCheryl A Kerfeld
Proceedings of the National Academy of Sciences of the United States of America
Chenguang FanThomas A Bobik
© 2022 Meta ULC. All rights reserved