De novo transcriptome analysis and gene expression profiling of an oleaginous microalga Scenedesmus acutus TISTR8540 during nitrogen deprivation-induced lipid accumulation

Scientific Reports
Anchalee SirikhachornkitPiyada Juntawong

Abstract

Nitrogen deprivation (-N) has been used as a technique to promote lipid accumulation in various microalgae. Scenedesmus acutus is a promising oleaginous green microalga that can be cultivated in organic wastewater for biodiesel production. Nevertheless, the molecular mechanisms controlling S. acutus lipid accumulation in response to -N remain unidentified. Physiological study determined that -N reduced cell growth and photosynthetic pigments. On the other hand, it promoted carbohydrate and neutral lipid accumulation. To find the mechanisms underlying lipid accumulation, we performed de novo transcriptome profiling of the non-model S. acutus in response to -N. The transcriptome analysis revealed that glycolysis and starch degradation were up-regulated; on the contrary, gluconeogenesis, photosynthesis, triacylglycerol (TAG) degradation and starch synthesis were down-regulated by -N. Under -N, the carbon flux was shifted toward fatty acid and TAG synthesis, and the down regulation of TAG lipase genes may contribute to TAG accumulation. A comparative analysis of the -N transcriptomes of oleaginous microalgae identified that the down-regulation of multiple lipase genes was a specific mechanism found only in the -N transcriptome of S...Continue Reading

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Citations

May 15, 2021·PloS One·Rachel A Schomaker, Jeffry L Dudycha

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Datasets Mentioned

BETA
SRR5894890
GFUP00000000

Methods Mentioned

BETA
RNA-seq
genetic modification
gene knockout
PCR

Software Mentioned

BLAST
BLASTX
PAGEMAN
Trinity
Blast2GO
MAPMAN
Trimmomatic
Bowtie2
R
Mercator

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