De Novo transcriptome analysis of Oncomelania hupensis after molluscicide treatment by next-generation sequencing: implications for biology and future snail interventions

PloS One
Qin Ping ZhaoHui Fen Dong

Abstract

The freshwater snail Oncomelania hupensis is the only intermediate host of Schistosoma japonicum, which causes schistosomiasis. This disease is endemic in the Far East, especially in mainland China. Because niclosamide is the only molluscicide recommended by the World Health Organization, 50% wettable powder of niclosamide ethanolamine salt (WPN), the only chemical molluscicide available in China, has been widely used as the main snail control method for over two decades. Recently, a novel molluscicide derived from niclosamide, the salt of quinoid-2',5-dichloro-4'-nitro-salicylanilide (Liu Dai Shui Yang An, LDS), has been developed and proven to have the same molluscicidal effect as WPN, with lower cost and significantly lower toxicity to fish than WPN. The mechanism by which these molluscicides cause snail death is not known. Here, we report the next-generation transcriptome sequencing of O. hupensis; 145,008,667 clean reads were generated and assembled into 254,286 unigenes. Using GO and KEGG databases, 14,860 unigenes were assigned GO annotations and 4,686 unigenes were mapped to 250 KEGG pathways. Many sequences involved in key processes associated with biological regulation and innate immunity have been identified. After t...Continue Reading

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Citations

Oct 21, 2016·PLoS Neglected Tropical Diseases·James J CodyMichael H Hsieh
Mar 23, 2017·Developmental and Comparative Immunology·Jonathan H Schultz, Coen M Adema

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Datasets Mentioned

BETA
SRP041729

Methods Mentioned

BETA
chromosomal aberrations
Assay
PCR
Illumina sequencing
RNA-Seq
cDNA library construction
electrophoresis
chemical treatment

Software Mentioned

Velvet
WEGO
SPSS
Blast2GO
EMBOSS
R ( edgeR )
Expectation - Maximization ( RSEM )

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