De novo transcriptome analysis unravels tissue-specific expression of candidate genes involved in major secondary metabolite biosynthetic pathways of Plumbago zeylanica: implication for pharmacological potential.

3 Biotech
Balachandran Karpaga Raja SundariS P Thyagarajan

Abstract

The present study provides comparative transcriptome analysis, besides identifying functional secondary metabolite genes of Plumbago zeylanica with pharmacological potential for future functional genomics, and metabolomic engineering of secondary metabolites from this plant towards diversified biomedical applications. Plumbago zeylanica is a widely used medicinal plant of the traditional Indian system of medicine with wide pharmacological potential to treat several disorders. The present study aimed to carry out comparative transcriptome analysis in leaf and root tissue of P. zeylanica using Illumina paired end sequencing to identify tissue-specific functional genes involved in the biosynthesis of secondary metabolites, contributing to its therapeutic efficacy. De novo sequencing assembly resulted in the identification of 62,321 "Unigenes" transcripts with an average size of 1325 bp. Functional annotation using BLAST2GO resulted in the identification of 50,301 annotated transcripts (80.71%) and GO assigned to 18,814 transcripts. KEGG pathway annotation of the "Unigenes" revealed that 2465 transcripts could be assigned to 242 KEGG pathway maps wherein the number of transcripts involved in secondary metabolism was distinct in roo...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 18, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T ThielA Graner
Dec 19, 2003·Nucleic Acids Research·Evelyn CamonRolf Apweiler
Feb 12, 2004·Bioinformatics·M J L de HoonS Miyano
Jun 8, 2004·Bioinformatics·Alok J Saldanha
Apr 30, 2005·Current Opinion in Plant Biology·Dianna BowlesBrigitte Poppenberger
Apr 8, 2006·Current Opinion in Plant Biology·Dorothea Tholl
Feb 19, 2008·Plant Physiology and Biochemistry : PPB·Joachim StöckigtElke A Loris
Jan 19, 2010·Journal of Ethnopharmacology·José-Luis Ríos
Sep 13, 2011·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·T T H DaoR Verpoorte
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jul 11, 2012·Asian Journal of Endoscopic Surgery·W D Lau, K Y Tan
Aug 24, 2012·Future Microbiology·Dianella Savoia
Sep 11, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Christudas SunilSavarimuthu Ignacimuthu
Oct 13, 2012·Frontiers in Plant Science·María L Falcone FerreyraPaula Casati
Nov 13, 2012·Bioinformation·Santosh Kumar Ramachandra JadhavBashir Mohammad Khan
Dec 5, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Shanmugam Hemaiswarya, Mukesh Doble
Mar 14, 2013·Current Opinion in Plant Biology·Charles StewartJoseph P Noel
Jan 29, 2014·TheScientificWorldJournal·Shashank Kumar, Abhay K Pandey
May 16, 2014·Journal of Natural Products·Dinesh Raghu, Devarajan Karunagaran
Dec 17, 2014·Plant Physiology·Craig Schluttenhofer, Ling Yuan
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Aug 19, 2015·Frontiers in Plant Science·Wei ZhuJingkui Tian
Jun 25, 2016·Plants·Nan JiangErich Grotewold
Oct 19, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Derong LinSaiyan Chen
Nov 24, 2016·Frontiers in Plant Science·Sukhdev SinghAshok Kumar
Dec 3, 2016·Nucleic Acids Research·Minoru KanehisaKanae Morishima
Feb 22, 2017·Frontiers in Plant Science·Reyhaneh Rahnamaie-TajadodNormah M Noor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.