Nov 4, 2018

De novo transcriptome assembly of the Italian white truffle (Tuber magnatum Pico)

BioRxiv : the Preprint Server for Biology
Federico VitaEdoardo Bertolini

Abstract

The Italian white truffle ( Tuber magnatum Pico) is a gastronomic delicacy that dominates the worldwide truffle market. Despite its importance, the genomic resources currently available for this species are still limited. Here we present the first de novo transcriptome assembly of T. magnatum . Illumina RNA-seq data were assembled using a single-k-mer approach into 22,932 transcripts with N50 of 1,524 bp. Our approach allowed to predict and annotate 12,367 putative protein coding sequences, reunited in 6,723 loci. In addition, we identified 2,581 gene-based SSR markers. This work provides the first publicly available reference transcriptome for genomics and genetic studies providing insight into the molecular mechanisms underlying the biology of this important species.

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Mentioned in this Paper

Biological Markers
Genome
Gene Delivery Systems
Genomics
Genus Tuber (fungus)
Genetic Studies
Species
Open Reading Frames
Transcriptome
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