De novo transcriptome of the diatom Cylindrotheca closterium identifies genes involved in the metabolism of anti-inflammatory compounds.

Scientific Reports
Ali M ElagozChiara Lauritano

Abstract

Diatoms are the most diverse and abundant group of phytoplankton species and represent a huge reservoir of marine natural products with possible application for human health. Several diatoms are known to have anticancer, anti-inflammatory, antioxidant and anti-microbial properties, but the compounds responsible of these activities are often still unknown. The diatom Cylindrotheca closterium showed anti-inflammatory properties inhibiting TNFα release in human monocytic leukemia cells. In this study, we present the full transcriptome of C. closterium, and used an -omic approach to identify transcripts coding enzymes that can be involved in the synthesis/degradation of anti-inflammatory compounds. This approach allowed to identify phosphatidylinositol-3-phosphatase, phosphatidylinositol 3-kinase catalytic subunit type 3, phosphatidylinositol N-acetylglucosaminyltransferase subunit A, monogalactosyldiacylglycerol synthase and violaxanthin de-epoxidase, which are known to be involved in anti-inflammatory compound metabolism. When C. closterium was cultured in silica-starvation conditions, selected as stress condition to potentially trigger the synthesis of bioactive metabolites, anti-inflammatory activity was lost and expression lev...Continue Reading

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Citations

May 20, 2020·Marine Drugs·Assunta SaideAdrianna Ianora
May 7, 2020·Marine Drugs·Gennaro RiccioChiara Lauritano
Oct 22, 2020·Marine Drugs·Chiara Lauritano, Adrianna Ianora
Jan 26, 2021·Bioresource Technology·Raya BhattacharjyaShireesh Srivastava
May 1, 2021·International Journal of Molecular Sciences·Assunta SaideChiara Lauritano
Jul 3, 2021·Biomedicines·Assunta SaideChiara Lauritano
Nov 10, 2021·Molecular Biology Reports·Che Lah Wan AfifudeenThye San Cha

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Datasets Mentioned

BETA
PRJNA577416

Methods Mentioned

BETA
RNA-seq
RNAseq
protein
PCR

Software Mentioned

Express
GraphPad
PHYRE2
Blast2GO
GraphPad Prim Statistic
geNorm
FastQC
EBSeq
REST tool
InterProScan

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