De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

Frontiers in Plant Science
Paulina GlazinskaJan Kopcewicz

Abstract

Yellow lupine (Lupinus luteus L., Taper c.), a member of the legume family (Fabaceae L.), has an enormous practical importance. Its excessive flower and pod abscission represents an economic drawback, as proper flower and seed formation and development is crucial for the plant's productivity. Generative organ detachment takes place at the basis of the pedicels, within a specialized group of cells collectively known as the abscission zone (AZ). During plant growth these cells become competent to respond to specific signals that trigger separation and lead to the abolition of cell wall adhesion. Little is known about the molecular network controlling the yellow lupine organ abscission. The aim of our study was to establish the divergences and similarities in transcriptional networks in the pods, flowers and flower pedicels abscised or maintained on the plant, and to identify genes playing key roles in generative organ abscission in yellow lupine. Based on de novo transcriptome assembly, we identified 166,473 unigenes representing 219,514 assembled unique transcripts from flowers, flower pedicels and pods undergoing abscission and from control organs. Comparison of the cDNA libraries from dropped and control organs helped in ident...Continue Reading

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Citations

Feb 15, 2020·International Journal of Molecular Sciences·Zhi-Lang QiuXiao-Peng Wen
Mar 12, 2020·International Journal of Molecular Sciences·Katarzyna Marciniak, Krzysztof Przedniczek
Aug 15, 2019·Journal of Applied Genetics·Sandra Rychel, Michał Książkiewicz

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Datasets Mentioned

BETA
PRJNA285604
SRX1069734
MI0021629

Methods Mentioned

BETA
RNA-Seq
RNA Seq
electrophoresis
transgenic
using

Software Mentioned

Bioconductor
R Development Core Team
Search miRBase
BLAST
R
RSEM
RNAstructure
plantGSEA
KAAS
Python

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