De novo transcriptomic analysis of the venomous glands from the scorpion Heterometrus spinifer revealed unique and extremely high diversity of the venom peptides

Toxicon : Official Journal of the International Society on Toxinology
Yuchen DengLiang Xiao

Abstract

Scorpion, as an ancient species, has been widely used on dozens of human diseases in traditional Chinese Medicine. Although the scorpion venom from the Buthidae family with the potent toxicity attracts more interests, toxins from the non-Buthidae family draw great attention as well because of its abundance and complexity even without harm to mammals. Moreover, several toxic components of scorpion venom have been identified as valuable scaffolds for the drug design and development. Using the Next Generation Sequencing (NGS) technique, here we reported the transcriptome of the venomous glands of Heterometrus spinifer, a non-Buthidae scorpion that only a few toxic and complete components have been identified known-to-date. The total mRNA extracted from the venomous glands of H. spinifer was subjected to illumina sequencing with a strategy of de novo assembly, and a total of 54 189 transcripts were unigenes from a total of 88 311 600 determined reads. We annotated 18 567 (34.26%) unigenes from NR database, 12 258 (22.62%) from SWISSPROT database, 11 161 (20.60%) from GO database, 10 159 (18.75%) from COG database and 5059 (9.34%) from KEGG database, respectively. 2843 unigenes were further selected against the toxin-related sub-dat...Continue Reading

Citations

Dec 14, 2019·Expert Review of Proteomics·Jimena I Cid-UribeLourival D Possani
Aug 15, 2019·BMC Genomics·Dwin G B GrashofArie van der Meijden
Dec 20, 2020·Toxicon : Official Journal of the International Society on Toxinology·Masahiro MiyashitaHisashi Miyagawa
Mar 4, 2020·Toxicon : Official Journal of the International Society on Toxinology·Laura L Valdez-VelázquezLourival D Possani
Jun 23, 2020·Toxicon : Official Journal of the International Society on Toxinology·Wen ZhuZongyun Chen

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