Deacetylase activity-independent transcriptional activation by HDAC2 during TPA-induced HL-60 cell differentiation

PloS One
Hyeonsoo JungSang-Beom Seo

Abstract

The human myeloid leukemia cell line HL-60 differentiate into monocytes following treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA). However, the mechanism underlying the differentiation of these cells in response to TPA has not been fully elucidated. In this study, we performed ChIP-seq profiling of RNA Pol II, HDAC2, Acetyl H3 (AcH3), and H3K27me3 and analyzed differential chromatin state changes during TPA-induced differentiation of HL-60 cells. We focused on atypically active genes, which showed enhanced H3 acetylation despite increased HDAC2 recruitment. We found that HDAC2 positively regulates the expression of these genes in a histone deacetylase activity-independent manner. HDAC2 interacted with and recruited paired box 5 (PAX5) to the promoters of the target genes and regulated HL-60 cell differentiation by PAX5-mediated gene activation. Taken together, these data elucidated the specific-chromatin status during HL-60 cell differentiation following TPA exposure and suggested that HDAC2 can activate transcription of certain genes through interactions with PAX5 in a deacetylase activity-independent pathway.

References

Jan 19, 2002·The Journal of Biological Chemistry·Alexander V EmelyanovBarbara K Birshtein
Oct 29, 2002·Nature Genetics·Oksana R BereshchenkoRiccardo Dalla-Favera
Sep 25, 2004·Journal of Cellular Physiology·Maria L MachedaJames D Best
Jul 12, 2005·Oncogene·Stefania MiccadeiDonato Civitareale
Aug 12, 2008·Seminars in Oncology·Christoph PlassGuido Marcucci
Jan 14, 2009·Laboratory Investigation; a Journal of Technical Methods and Pathology·Rajani KantetiRavi Salgia
Feb 18, 2009·Blood·Daniel NowakH Phillip Koeffler
May 27, 2009·Current Opinion in Hematology·Kevin PetrieSamuel Waxman
Oct 14, 2009·Blood·Erin A KimbrelAndrew L Kung
Jan 12, 2011·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Alan BurnettBob Löwenberg
Sep 14, 2011·The Journal of Experimental Medicine·Christian HurtzMarkus Müschen
Oct 6, 2011·Advances in Immunology·Jasna MedvedovicMeinrad Busslinger
May 23, 2012·Molecular and Cellular Biology·Ji-Young KimSang-Beom Seo
Jul 10, 2012·Cell·Mark A Dawson, Tony Kouzarides
Jul 18, 2012·Molecular and Cellular Biology·Hye-Ju SonSang-Beom Seo
Dec 12, 2012·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Tomas KupsaLadislav Jebavy
Dec 19, 2012·Immunologic Research·Sonia I VlaicuHorea Rus
May 3, 2013·The New England Journal of Medicine·UNKNOWN Cancer Genome Atlas Research NetworkGreg Eley
Mar 4, 2015·Leukemia·W BéguelinR Shaknovich
Mar 17, 2015·Experimental and Molecular Pathology·Cosmin A TeglaHorea Rus
Sep 17, 2015·The New England Journal of Medicine·Hartmut DöhnerClara D Bloomfield
Jun 17, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Frank G RückerLars Bullinger
Apr 4, 2017·Cell Systems·Ricardo N RamirezAli Mortazavi

❮ Previous
Next ❯

Citations

Dec 18, 2019·Journal of the American Society of Nephrology : JASN·Long ZhangWenzheng Zhang
May 22, 2019·Molecular Cancer Research : MCR·Jordan S ShafranGerald V Denis

❮ Previous
Next ❯

Methods Mentioned

BETA
immunoprecipitation
ChIP
ChIP-seq
PCR
FACS
histone acetylation
acetylation

Software Mentioned

TreeView
Bowtie
cisRED
Excel
UCSC
HOMER
Java

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.