Abstract
Acemannan, an acetylated polymannose from Aloe vera, induces tissue repair. We investigated the role of acemannan's acetyl-groups on its physical and biological properties. Deacetylated acemannan (DeAcAM) was prepared and characterized. The physical properties and microscopic structure of DeAcAM were evaluated using water solubility, contact angle, X-ray diffraction, and scanning-electron microscopy. The activity of DeAcAM on cell proliferation and gene expression were assessed. Acemannan and DeAcAM structures were simulated and the acemannan tetramer diad and its completely deacetylated structure were also determined. Increased acemannan deacetylation reduced its water solubility and hydrophilicity. Complete deacetylation altered acemannan's conformation to a partial crystal structure. The bioactivity of acemannan was reduced corresponding to its deacetylation. Acemannan induced cell proliferation, and VEGF and Collagen I expression; however, 100% DeAcAM did not. The simulated structures of the acemannan diad and the completely deacetylated diad were different. We conclude acetyl-groups affect acemannan's structure and physical/biological properties.
References
Mar 1, 1979·Nucleic Acids Research·A V VologodskiiM D Frank-Kamenetskii
Oct 29, 1992·Nature·D ShweikiE Keshet
Mar 1, 1995·International Journal of Immunopharmacology·K KaracaR Nordgren
Mar 30, 2001·Periodontology 2000·I Aukhil
Jul 20, 2001·International Immunopharmacology·J K LeeC K Lee
Aug 21, 2001·The Journal of Investigative Dermatology·G SzolnokyL Kemény
Aug 9, 2003·Journal of Pharmaceutical and Biomedical Analysis·M LavertuAjay Gupta
Mar 27, 2004·Physiological Reviews·Michael Kjaer
May 20, 2004·Cancer Research·Heidemarie RossiterErwin Tschachler
Nov 9, 2004·International Immunopharmacology·Carlton E TurnerDoug J Talley
Mar 31, 2005·Carbohydrate Research·Jimmy Tai-Nin ChowWarren J Goux
Nov 18, 2005·Gene·Michele A GlozakEdward Seto
Jan 18, 2006·International Journal of Pharmaceutics·K S C R dos SantosM H Gil
May 9, 2008·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenlin XuJie Pang
Nov 26, 2008·The Journal of Surgical Research·Philip BaoHarold Brem
Apr 18, 2009·Journal of Pharmacological Sciences·Suwimon JettanacheawchankitPasutha Thunyakitpisal
Jul 19, 2012·The Journal of Biological Chemistry·Rui ChenJoseph A Garcia
Dec 13, 2012·International Journal of Oncology·Atsushi MatsumuraEigo Otsuji
May 29, 2013·Journal of Periodontal Research·P ChantarawaratitP Thunyakitpisal
Mar 4, 2014·Current Opinion in Genetics & Development·Nick Gilbert, James Allan
Sep 1, 2012·Carbohydrate Polymers·Joana SimõesM Rosário Domingues
Apr 29, 2014·Bioorganic Chemistry·Patrick J MoynihanAnthony J Clarke
Aug 27, 2014·International Journal of Biological Macromolecules·Haiping LiLihua Huai
Citations
Nov 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoli WangPengfei Liu
Mar 15, 2019·Food & Function·Liu-Jing ZhangShao-Ping Nie
Apr 24, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chang LiuHe Qian
Oct 1, 2019·Implant Dentistry·Hai Anh TrinhPasutha Thunyakitpisal
Jan 1, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Solomon Habtemariam
Apr 4, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Zaira LópezPeter Knauth
Apr 10, 2019·Polymers·Daniel Alonso Miramon-OrtízJaime Lizardi-Mendoza
Sep 3, 2020·International Journal of Molecular Sciences·Can SiJun Duan
Jul 12, 2020·Pharmaceutics·Tien Thuy VuPasutha Thunyakitpisal
Feb 22, 2017·Journal of Functional Biomaterials·Shekh RahmanNarayan Bhattarai
May 30, 2019·Frontiers in Plant Science·Louise Isager AhlNina Rønsted
Aug 28, 2017·Polymers·Can JinKan Ding
Dec 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jiao XuePing Liu
Oct 18, 2020·Tissue Engineering. Part B, Reviews·Jiaheng LiangJingan Li
Jan 30, 2021·PloS One·Mengjian LiuJunli Niu
Mar 13, 2021·Food & Function·Yu-Jing WangJian-Ping Luo
Mar 27, 2021·Carbohydrate Polymers·Chang LiuHe Qian
Apr 17, 2021·International Journal of Biological Macromolecules·Dharunya GovindarajanManikantan Syamala Kiran
Aug 20, 2018·International Journal of Biological Macromolecules·Kiran PatruniSrinivasa Rao Pavuluri
Jul 24, 2021·Carbohydrate Polymers·Guangying YeZaihua Wang
Jul 13, 2021·Protoplasma·Can SiJun Duan
Aug 15, 2021·Scientific Reports·Ajeeta AnandHideki Aoyagi
Aug 19, 2021·Chembiochem : a European Journal of Chemical Biology·Robert LassfolkReko Leino
May 19, 2019·Ultrasonics Sonochemistry·Guadalupe Alvarado-MoralesAntoni Femenia
Dec 19, 2019··Hana N. UlyaDyah H. Wardhani
Jun 16, 2017··Mochammad A. AshidiqAji Prasetyaningrum