Deactivation kinetics of different components of calcium inward current in the membrane of mice sensory neurones.

The Journal of Physiology
P G Kostyuk, R E Shirokov

Abstract

1. Deactivation kinetics of different components of calcium inward current were analysed by measuring 'tail currents' in intracellularly perfused sensory neurones isolated from dorsal root ganglia of newborn mice. 2. Deactivation of a low-threshold inactivating component had a mono-exponential time course with time constants that decreased as the potential was made more negative, and reached a limiting value of 1.0-1.2 ms at extreme hyperpolarizations. Replacement of Ca2+ by Ba2+ as a charge carrier decreased this value almost twofold. 3. Deactivation of the high-threshold component of calcium current contained two exponential components with time constants of similar potential dependence. The fast time constant became practically constant (0.1-1.2 ms) during repolarizations beyond -50 mV, and the slow time constant became constant (0.80-0.85 ms) at repolarization beyond -70 mV. 4. Replacement of intracellular aspartate with phosphate, or a depolarized holding potential of -40 mV, abolished reversibly the slow component of the tail current; in parallel, the peak current-voltage relation of the current became shifted by 15-20 mV in the depolarizing direction. 5. Extracellular application of the calcium channel agonist Bay K 8644...Continue Reading

Citations

Oct 24, 2013·Pflügers Archiv : European journal of physiology·Chris BladenGerald W Zamponi
Apr 1, 1999·Annual Review of Physiology·B L Sabatini, W G Regehr
Dec 31, 2002·Physiological Reviews·Edward Perez-Reyes
Sep 13, 2001·The Journal of Toxicological Sciences·M OkudaT Hobara

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