Deactivation mechanism of the green fluorescent chromophore

The Journal of Physical Chemistry. B
Rinat GepshteinNoam Agmon

Abstract

We report time-resolved fluorescence data for the anion of p-hydroxybenzylidene dimethylimidazolinone (p-HBDI), a model chromophore of the green fluorescence protein, in viscous glycerol-water mixtures over a range of temperatures, T. The markedly nonexponential decay of the excited electronic state is interpreted with the aid of an inhomogeneous model possessing a Gaussian coordinate-dependent sink term. A nonlinear least-squares fitting routine enables us to achieve quantitative fits by adjusting a single activation parameter, which is found to depend linearly on 1/T. We derive an analytic expression for the absolute quantum yield, which is compared with the integrated steady-state fluorescence spectra. The microscopic origins of the model are discussed in terms of two-dimensional dynamics, coupling the phenyl-ring rotation to a swinging mode that brings this flexible molecule to the proximity of a conical intersection on its multidimensional potential energy surface.

References

Dec 2, 1975·Biochemistry·R H AustinI C Gunsalus
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·H NiwaF I Tsuji
Jan 22, 1998·Current Opinion in Structural Biology·G N Phillips
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·W WeberP W Langhoff
Apr 29, 2004·Journal of the American Chemical Society·María Elena MartinMassimo Olivucci
Feb 1, 2005·Biophysical Journal·Noam Agmon

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Citations

Aug 25, 2011·Accounts of Chemical Research·Laren M TolbertKyril M Solntsev
Jun 29, 2012·Accounts of Chemical Research·Nadav AmdurskyDan Huppert
Jun 30, 2011·The Journal of Physical Chemistry. a·Yuval ErezDan Huppert
Oct 15, 2011·The Journal of Physical Chemistry. a·Shahnawaz RafiqGurunath Ramanathan
Mar 29, 2013·The Journal of Physical Chemistry. a·Michael WalterMichael Moseler
Oct 30, 2009·Physical Chemistry Chemical Physics : PCCP·J RajputM Brøndsted Nielsen
Jun 29, 2010·Physical Chemistry Chemical Physics : PCCP·A TrivellaS Coussan
Feb 7, 2012·Physical Chemistry Chemical Physics : PCCP·Rolando R Lozada-GarcíaClaudine Crépin
Sep 4, 2012·Physical Chemistry Chemical Physics : PCCP·Yue-Jie AiYi Luo
Sep 23, 2009·Chemical Society Reviews·Stephen R Meech
Jun 30, 2011·Protein Science : a Publication of the Protein Society·S James Remington
Mar 26, 2015·Physical Chemistry Chemical Physics : PCCP·Russell B VeghKyril M Solntsev
Dec 22, 2014·The Journal of Chemical Physics·Li ZhaoKe-Li Han
Oct 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ziad Ganim, Matthias Rief
Apr 30, 2019·Physical Chemistry Chemical Physics : PCCP·Miles A TaylorChong Fang
Nov 20, 2019·Nucleic Acids Research·Christian SteinmetzgerClaudia Höbartner
Nov 30, 2019·The Journal of Chemical Physics·Chong FangCheng Chen
May 22, 2013·Chemical Communications : Chem Comm·Mikhail S BaranovIlia V Yampolsky
Jul 21, 2006·The Journal of Physical Chemistry. a·Pavel LeidermanDan Huppert
Feb 14, 2008·The Journal of Physical Chemistry. B·Kyril M SolntsevLaren M Tolbert
May 22, 2008·The Journal of Physical Chemistry. B·R GepshteinDan Huppert
Mar 7, 2009·The Journal of Physical Chemistry. a·Shigeo KanekoSeiji Tobita
Oct 2, 2008·Journal of the American Chemical Society·Jian DongLaren M Tolbert
Dec 17, 2009·The Journal of Physical Chemistry. a·Irina PetkovaEric Vauthey
Jun 3, 2021·International Journal of Molecular Sciences·Sean R TachibanaChong Fang
Jul 3, 2021·Physical Chemistry Chemical Physics : PCCP·Sean A BoulangerChong Fang
Aug 16, 2012·The Journal of Physical Chemistry Letters·Kiri AddisonStephen R Meech
Feb 18, 2011·Journal of the American Chemical Society·Cheng-Chih HsiehTaiha Joo
Jan 28, 2011·The Journal of Physical Chemistry. B·Jamie ConyardStephen R Meech
Mar 9, 2011·The Journal of Physical Chemistry. a·Nadav AmdurskyDan Huppert
Dec 18, 2019·Journal of the American Chemical Society·Soumit ChatterjeePeter Karuso
Sep 3, 2021·Physical Chemistry Chemical Physics : PCCP·Joanne L WoodhouseHelen H Fielding
Jan 10, 2008·The Journal of Physical Chemistry. B·P LeidermanDan Huppert
Apr 28, 2006·The Journal of Physical Chemistry. a·Pavel LeidermanDan Huppert

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