PMID: 6990237Feb 1, 1980Paper

Deactivation of furyl furamide (AF-2) by rat-liver microsomes and its implication in short-term tests for mutagenicity/carcinogenicity

Mutation Research
M S Murthy, K B Najaria

Abstract

The genetic activity of AF-2 in both bacteria and yeast rapidly disappeared in the presence of rat-liver microsomal fraction (S9 mix). Incubation of AF-2 with S9 mix even for 10 min at 37 degrees C was sufficient to inactivate it completely. Data available in the literature suggest that activation of AF-2 is necessary for its geno-toxic effect. The activation step may involve reduction of the nitrofuran to an amino group probably by the enzyme reductase I. Most cultured cell systems, such as bacteria, yeast, Neurospora, mammalian cells and human lymphocytes, can probably bring about this reduction. However, the rapid disappearance of the genetic activity of AF-2 in the presence of rat-liver homogenate suggests that rat-liver microsomes may further metabolize the reduction products to inactive forms. Thus, it becomes necessary to test even those chemicals that are mutagenic per se, with mammalian microsomal preparations before their mutagenic/carcinogenic potentialities can be assessed in short-term tests.

References

Oct 1, 1978·Mutation Research·F L Petrilli, S De Flora
Jul 1, 1976·Biochemical Pharmacology·H S Rosenkranz, W T Speck
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Aug 1, 1975·Mutation Research·E R Soares, W Sheridan
Jan 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·S Fogel, R K Mortimer

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Citations

Dec 12, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jorge I González BorrotoRicard Marcos
Oct 29, 2010·Mutagenesis·John A HeddleJames T MacGregor

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