PMID: 6396Jan 1, 1976

Deamidated active intermediates in the irreversible acid denaturation of ribonuclease-A

International Journal of Peptide and Protein Research
B N ManjulaP J Vithayathil

Abstract

A study has been made on the changes in the enzymatic activity of Ribonuclease-A**-(RNase-A) exposed to highly acidic (pH less than 1) acqueous environment. Irreversible alterations of activity were observed when the protein was exposed to an acidic medium for a long period (20 to 60 h). Even prior to these changes in activity RNase-A was found to form intermediates which had very nearly the same activity as the native protein. The primary process in the acid denaturation of RNase-A was observed to be deamidation of the protein leading to the formation of active chromotographically distinct derivatives. The initial product of deamidation, a monodeamidated derivative, has been isolated by chromatography on Amberlite XE-64. This initial deamidation reaction proceeded with very high specificity. The subsequent deamidation reaction is comparatively slower, so that nearly 50% of the native protein could be converted to this derivative before any subsequent deamidation took place. This monodeamidated derivative has been designated RNase-Aa1. The conversion of RNase-A to RNase-Aa1 was not accompanied by any changes in the primary structure other than the observed deamidation. Apart from the differences in chromatographic and electroph...Continue Reading

References

Jan 1, 1975·International Journal of Peptide and Protein Research·A S Acharya, P J Vithayathil
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Citations

Jan 1, 1977·International Journal of Peptide and Protein Research·A S AcharyaP J Vithayathil
Jan 1, 1985·International Journal of Peptide and Protein Research·Y P Venkatesh, P J Vithayathil
Jan 1, 1981·International Journal of Peptide and Protein Research·S Ramnath, P J Vithayathil
Nov 1, 1978·International Journal of Peptide and Protein Research·M K Das, P J Vithayathil
May 1, 1984·International Journal of Peptide and Protein Research·Y P Venkatesh, P J Vithayathil
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·H T Wright

Related Concepts

Acids
Ammonia
Chromatography
Electrophoresis, Disc
Hydrogen-Ion Concentration
Protein Conformation
Protein Denaturation
Alkaline Ribonuclease

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