Deamination of amino acids as a source for ammonia production in human skeletal muscle during prolonged exercise

The Journal of Physiology
G van HallA J Wagenmakers

Abstract

1. The influence of pre-exercise muscle glycogen content on ammonia production, adenine nucleotide breakdown and amino acid metabolism was investigated during prolonged exercise in six subjects having one leg with a normal and one leg with a low muscle glycogen content. One-leg knee-extensor exercise was performed for 90 min, at a workload of 60-65% of the maximal power output, first with one leg and then with the other. 2. During exercise ammonia was released in gradually increasing amounts and plateaued after 1 h exercise at a rate of approximately 80 mumol min-1. The total ammonia production was 9.1 +/- 0.4 and 9.5 +/- 1.4 mmol (kg dry muscle)-1 in the normal and low glycogen content leg, respectively. 3. Levels of muscle phosphocreatine (PC), total adenine nucleotides and inosine monophosphate (IMP) were similar at rest and after 90 min of exercise. 4. Only minor differences were observed between rest and exercise and between legs for the muscle concentrations of glutamine, alanine and the branched-chain amino acids. Muscle glutamate concentration decreased by 60-70% within the first 10 min of exercise. Glutamate consumption over 90 min quantitatively equalled ammonia production. Most of the glutamate was consumed within th...Continue Reading

Citations

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