Death induction by recombinant native TRAIL and its prevention by a caspase 9 inhibitor in primary human esophageal epithelial cells.

The Journal of Biological Chemistry
Seok-Hyun KimWafik S El-Deiry

Abstract

The cytotoxic death ligand TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a tumor-specific agent under development as a novel anticancer therapeutic agent. However, some reports have demonstrated toxicity of certain TRAIL preparations toward human hepatocytes and keratinocytes through a caspase-dependent mechanism that involves activation of the extrinsic death pathway and Type II signaling through the mitochondria. We have isolated and purified both His-tagged protein and three versions of native recombinant human TRAIL protein from Escherichia coli. We found that 5 mm dithiothreitol in the purification process enhanced oligomerization of TRAIL and resulted in the formation of hyper-oligomerized TRAILs, including hexamers and nonomers with an extremely high potency in apoptosis induction. Although death-inducing signaling complex formation was much more efficient in cells treated with hyper-oligomerized TRAILs, this did not convert TRAIL-sensitive Type II HCT116 colon tumor cells to a Type I death pattern as judged by their continued sensitivity to a caspase 9 inhibitor. Moreover, TRAIL-resistant Type II Bax-null colon carcinoma cells were not converted to a TRAIL-sensitive Type I state by hyper-oligomerize...Continue Reading

References

Apr 4, 1997·Science·G PanV M Dixit
Oct 6, 1997·The EMBO Journal·H WalczakC T Rauch
Oct 7, 1997·The Journal of Experimental Medicine·M A Degli-EspostiC A Smith
Nov 5, 1997·Nature Genetics·G S WuW S el-Deiry
Feb 28, 1998·The Journal of Biological Chemistry·M MuzioV M Dixit
Aug 28, 1998·Science·D R Green, J C Reed
Jun 8, 1999·Annual Review of Immunology·D WallachM P Boldin
Jul 20, 1999·The Journal of Clinical Investigation·A AshkenaziR H Schwall
Jan 7, 2000·The American Journal of Pathology·F MeierM Herlyn
Mar 10, 2000·Biochimica Et Biophysica Acta·H R Stennicke, G S Salvesen
May 10, 2000·Nature Medicine·S Nagata
Apr 3, 2001·Nature Medicine·D LawrenceA Ashkenazi
Dec 12, 2001·Molecular and Cellular Biology·Alicia Algeciras-SchimnichMarcus E Peter
Apr 10, 2002·The Journal of Biological Chemistry·Youngsoo KimJohn C Reed
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Alicia Algeciras-SchimnichMarcus E Peter
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shulin Wang, Wafik S El-Deiry

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Citations

Apr 18, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ronald KoschnyTom M Ganten
Jan 16, 2010·Cancer Gene Therapy·H-Y ZhangA-L Li
Apr 30, 2005·Cell Death and Differentiation·M MacFarlaneG M Cohen
Jun 10, 2011·Science Translational Medicine·Nathan G DolloffWafik S El-Deiry
Mar 25, 2006·Future Oncology·Zodwa DlaminiThokozile Ledwaba
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Wenge WangWafik S El-Deiry
Jun 28, 2011·Gastroenterology·Joshua E Allen, Wafik S El-Deiry
Mar 10, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Rui LiXiaofeng Lu
Dec 31, 2005·Annals of the New York Academy of Sciences·Alan D BrooksThomas J Sayers
Jun 2, 2015·Cell Cycle·Mala K TalekarWafik S El-Deiry
Aug 19, 2006·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Esther H J WissinkMarcel Verheij
Jan 24, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Mamoun YounesAnas Younes
Mar 25, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kun ZhouSong Zhao
Sep 16, 2016·Apoptosis : an International Journal on Programmed Cell Death·Lei ChenYifa Zhou
Dec 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Eric K Rowinsky
Oct 20, 2017·Cell Death & Disease·Yelyzaveta ShlyakhtinaHinrich Gronemeyer
Dec 22, 2007·Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research·A M ChromikU Mittelkötter
Jun 13, 2014·International Journal of Oncology·Yoshifumi UmemoriNaoki Watanabe
Oct 25, 2017·Antibodies·Agathe Dubuisson, Olivier Micheau

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