PMID: 8581564Sep 1, 1995Paper

Death of cultured human neuroblastoma cells induced by HIV-1 gp120 is prevented by NMDA receptor antagonists and inhibitors of nitric oxide and cyclooxygenase

Neurodegeneration : a Journal for Neurodegenerative Disorders, Neuroprotection, and Neuroregeneration
M T CorasanitiG Nisticò

Abstract

The cytotoxic effects of the human immunodeficiency virus type 1 (HIV-1) coat protein gp120 were studied in human CHP100 neuroblastoma cell cultures. Incubation of neuroblastoma cultures with gp120 (1 pM-10 nM) induces cell death which is not concentration-related. The significant cell death evoked by 10 pM gp120 was prevented by neutralization of the viral protein with a monoclonal anti-gp120 (IgG) antibody. In addition, gp120-induced cytotoxicity was inhibited by [DL-(E)-2-amino-4-methyl-5-phosphono-3-pentenoic acid] (CGP37849; 100 microM), [(+/-)-3R*, 4as*, 6R*, 8aR*-6-(phosphonomethyl) decahydro-isoquinoline-3-carboxylic acid] (LY274614; 100 microM), MK801 (dizocilpine; 200 nM) and 7-chloro kynurenic acid (100 microM), selective antagonists of the NMDA receptor complex; by contrast, (6-cyano-7-nitroquinoxaline-2,3-dione (CNQX; 100 microM), a non-NMDA antagonist, was ineffective. Prevention of the lethality elicited by the HIV-1 coat protein was also obtained by incubating neuroblastoma cells with gp120 in Ca(2+)-free medium. The lethal effects induced by gp120 involve activation of L-arginine-nitric oxide (NO) pathway since these were prevented by haemoglobin (10 microM), a NO-trapping agent, and by D-arginine (1 mM), the l...Continue Reading

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Citations

Jun 10, 1998·Biochemical and Biophysical Research Communications·M T CorasanitiA Finazzi-Agrò
Jul 24, 2001·Medical and Pediatric Oncology·M Annicchiarico-PetruzzelliG Melino
Aug 9, 2001·Journal of Neuroscience Research·M NavarraG Di Renzo
Apr 5, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Michelle C PotterBarbara S Slusher
Jul 30, 2005·Journal of Biosciences·Hakan Ozdener
Oct 18, 2007·Applied Microbiology and Biotechnology·Etsuko KumagaiShinji Harada
Aug 18, 2012·Molecular Neurobiology·Giovanna De ChiaraAnna Teresa Palamara
Apr 13, 2004·Oncogene·Pierluigi Nicotera, Gerry Melino
Nov 21, 1998·The Journal of Biological Chemistry·M MaccarroneA F Agrò
Mar 8, 2011·Molecular Neurobiology·Richard KillickMassimiliano Agostini
Apr 16, 1998·Biochemical and Biophysical Research Communications·G BagettaA Finazzi-Agrò
Jul 14, 2000·Journal of Medicinal Chemistry·H Bräuner-OsborneP Krogsgaard-Larsen
Apr 9, 2005·Experimental Neurology·William A BanksAvindra Nath

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