Death rate in a small air-lift loop reactor of vero cells grown on solid microcarriers and in macroporous microcarriers.

Cytotechnology
D E MartensJ Tramper

Abstract

The death rate of Vero cells grown on Cytodex-3 microcarriers was studied as a function of the gas flow rate in a small air-lift loop reactor. The death rate may be described by first-order death-rate kinetics. The first-order death-rate constant as calculated from the decrease in viable cells, the increase in dead cells and the increase in LDH activity is linear proportional to the gas flow rate, with a specific hypothetical killing volume in which all cells are killed of about 2·10(-3) m(3) liquid per m(3) of air bubbles. In addition, an experiment was conducted in the same air-lift reactor with Vero cells grown inside porous Asahi microcarriers. The specific hypothetical killing volume calculated from this experiment has a value of 3·10(-4) m(3) liquid per m(3) of air bubbles, which shows that the porous microcarriers were at least in part able to protect the cells against the detrimental hydrodynamic forces generated by the bubbles.

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Citations

Aug 21, 2014·Biotechnology Progress·Gerco van EikenhorstWilfried A M Bakker
Jan 1, 2010·Biotechnology Progress·Maria Elisa RodriguesRosário Oliveira
Mar 27, 2018·Biotechnology and Bioengineering·Ashkan YekrangSafakarKidong Park
Jan 1, 1996·Cytotechnology·J TramperC D de Gooijer
Jan 1, 1996·Cytotechnology·J TramperC D de Gooijer
Mar 19, 2021·Critical Reviews in Biotechnology·Alonso Ornelas-GonzálezMarco Rito-Palomares

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