DEC2 expression antagonizes cisplatin‑induced apoptosis in human esophageal squamous cell carcinoma

Molecular Medicine Reports
Hidenobu SatoHiroshi Kijima

Abstract

Differentiated embryonic chondrocyte expressed gene 1 (DEC1) and differentiated embryonic chondrocyte expressed gene 2 (DEC2) belong to the Hairy/Enhancer of Split subfamily of basic helix‑loop‑helix factors. Previous studies have demonstrated that DEC proteins are involved in the regulation of circadian rhythms, response to hypoxia, and tumorigenesis. However, the roles of DEC1 and DEC2 in apoptosis of esophageal carcinoma remain unclear. In the present study, alterations in expression of apoptosis‑related markers in human esophageal squamous cell carcinoma TE‑11 cells treated with cisplatin were examined by western blot, while overall cell viability and apoptosis were analyzed by MTS assay and hematoxylin and eosin staining, respectively. Following cisplatin treatment, expression of DEC2 was downregulated, whereas expression of DEC1 was upregulated. DEC2 overexpression during cisplatin treatment markedly inhibited expression of the pro‑apoptotic factor Bim and slightly increased the anti‑apoptotic factor Bcl‑xL. However, overexpression of DEC1 during cisplatin treatment failed to affect expression of these markers. Additionally, overexpression of DEC2 improved cell viability and decreased cell apoptosis induced by cisplatin. ...Continue Reading

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Citations

Feb 20, 2020·Journal of Cellular and Molecular Medicine·Di ZhangEnhua Wang
Nov 30, 2018·Journal of Cellular Biochemistry·Hongyu LiYunshan Wang
Apr 4, 2021·Biology·Juan Alfonso RedondoBenjamin Beck

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Methods Mentioned

BETA
transfection

Software Mentioned

GraphPad
ImageJ
GraphPad Prism

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